{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T22:45:41Z","timestamp":1761518741900,"version":"build-2065373602"},"reference-count":27,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2004,4,8]],"date-time":"2004-04-08T00:00:00Z","timestamp":1081382400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2004,5,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Mass spectrometric methods, including matrix\u2010assisted laser desorption\/ionisation time\u2010of\u2010flight mass spectrometry (MALDI\u2010TOFMS), on\u2010line liquid chromatography\/electrospray ionisation mass spectrometry (LC\/ESI\u2010MS), and nanospray ionisation\/hybrid quadrupole time\u2010of\u2010flight mass spectrometry (nanoESI\u2010QqTOFMS), were applied to characterize by mass fingerprinting the venom of the French Guyanese tarantula\n                    <jats:italic>Theraphosa leblondi<\/jats:italic>\n                    . Of these techniques direct nanoESI\u2010QqTOFMS, which allowed the detection of 65 protonated molecules with high mass accuracy, appeared to give the best results. Three major peptides, TlTx1, TlTx2 and TlTx3, were sequenced using a combination of nanoESI\u2010MS\/MS and enzyme digestion\/MS and MS\/MS experiments. Each sequence was confirmed by automated Edman sequencing. In patch\u2010clamp experiments these peptides were found to have a specific inhibitory effect on the voltage\u2010dependent potassium channel, Kv4.2. Copyright \u00a9 2004 John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/rcm.1442","type":"journal-article","created":{"date-parts":[[2004,4,8]],"date-time":"2004-04-08T12:04:13Z","timestamp":1081425853000},"page":"1024-1032","source":"Crossref","is-referenced-by-count":24,"title":["Nanospray analysis of the venom of the tarantula\n                    <i>Theraphosa leblondi<\/i>\n                    : a powerful method for direct venom mass fingerprinting and toxin sequencing"],"prefix":"10.1002","volume":"18","author":[{"given":"Christian","family":"Legros","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie\u2010Louise","family":"C\u00e9l\u00e9rier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maud","family":"Henry","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Guette","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,4,8]]},"reference":[{"volume-title":"Advancesin Spider Taxonomy, 1988\u20131991","year":"1993","author":"Platnick NI","key":"e_1_2_6_2_2"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(01)00277-X"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-9084(00)01166-4"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(01)00199-4"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1999.00622.x"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1021836403433"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/mas.10062"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.2116\/analsci.19.3"},{"key":"e_1_2_6_10_2","first-page":"236","volume":"4","author":"Jurgens M","year":"2002","journal-title":"Curr. 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