{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T11:49:30Z","timestamp":1761479370379,"version":"build-2065373602"},"reference-count":26,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2004,5,14]],"date-time":"2004-05-14T00:00:00Z","timestamp":1084492800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2004,6,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    A recombinant peptidic spider toxin, HpTx2, was investigated directly by nanoelectrospray tandem mass spectrometry (MS\/MS). This 30\u2010residue toxin possesses a highly knotted structure with cystines arranged in close proximity. The low\u2010energy collision\u2010induced dissociation MS\/MS spectrum of the [M+4H]\n                    <jats:sup>4+<\/jats:sup>\n                    ion permitted characterization of the C\u2010terminal sequence of HpTx2 up to Cys\n                    <jats:sub>26<\/jats:sub>\n                    that is involved in a disulfide bridge. Chemical pre\u2010treatment with DTT or TCEP was then investigated, and it was found that an unexpected cleavage reaction of HpTx2 gave two smaller peptides which were completely sequenced by MS\/MS experiments using a Qq\u2010TOF mass spectrometer. This unusual hydrolysis reaction facilitated the determination of the complete sequence of the HpTx2 toxin. Copyright \u00a9 2004 John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/rcm.1490","type":"journal-article","created":{"date-parts":[[2004,5,14]],"date-time":"2004-05-14T11:01:06Z","timestamp":1084532466000},"page":"1317-1323","source":"Crossref","is-referenced-by-count":4,"title":["An unusual cleavage reaction of a peptide observed during dithiotreitol and tris(2\u2010carboxyethyl)phosphine reduction: application to sequencing of HpTx2 spider toxin using nanospray tandem mass spectrometry"],"prefix":"10.1002","volume":"18","author":[{"given":"Christian","family":"Legros","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie\u2010Louise","family":"C\u00e9l\u00e9rier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Guette","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,5,14]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(01)00199-4"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-003-3108-6"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2004.02.009"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/1522-2675(20010815)84:8<2178::AID-HLCA2178>3.0.CO;2-N"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-9084(00)01166-4"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/PL00000794"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0231(199711)11:17<1891::AID-RCM94>3.0.CO;2-X"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0231(19990930)13:18<1861::AID-RCM730>3.0.CO;2-7"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/mas.1280060102"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/mas.1280140104"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1044-0305(02)00441-5"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/379466a0"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac9509519"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(03)01426-8"},{"key":"e_1_2_6_16_2","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/S0026-895X(24)13491-8","volume":"51","author":"Sanguinetti MC","year":"1997","journal-title":"Mol. 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