{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T18:44:18Z","timestamp":1791053058428,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"19","license":[{"start":{"date-parts":[[2004,8,26]],"date-time":"2004-08-26T00:00:00Z","timestamp":1093478400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2004,10,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    High\u2010performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry (HPLC\/ESI\u2010MS\/MS) has been used to search for the formation of as yet unidentified radiation\u2010induced DNA lesions. For that purpose, the characteristic fragmentation of most of 2\u2032\u2010deoxyribonucleosides that corresponds to the loss of the 2\u2010deoxyribose moiety (loss of 116 mass units) has been utilized to specifically detect modified nucleosides. Aerated aqueous solutions of DNA were exposed to ionizing radiation, and subsequently DNA was digested to nucleosides with a cocktail of endo\u2010 and exonucleases. HPLC\/ESI\u2010MS\/MS analysis of the resulting 2\u2032\u2010deoxyribonucleoside mixture allowed us to detect four novel DNA modifications. In a subsequent step, the sensitivity of the tandem mass spectrometer was used to search for the formation of the newly detected lesions in the DNA of\n                    <jats:italic>\u03b3<\/jats:italic>\n                    \u2010irradiated cells. Thus, one of the four newly detected lesions was found to be significantly generated in cellular DNA upon exposure to ionizing radiation. In addition, the latter lesion was also shown to be present in untreated cells, indicating that the modified nucleoside could be formed endogenously. Copyright \u00a9 2004 John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/rcm.1612","type":"journal-article","created":{"date-parts":[[2004,8,26]],"date-time":"2004-08-26T09:49:01Z","timestamp":1093513741000},"page":"2223-2228","source":"Crossref","is-referenced-by-count":38,"title":["Detection of new radiation\u2010induced DNA lesions by liquid chromatography coupled to tandem mass spectrometry"],"prefix":"10.1002","volume":"18","author":[{"given":"Peggy","family":"Regulus","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9bastien","family":"Spessotto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathilde","family":"Gateau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean","family":"Cadet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain","family":"Favier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Luc","family":"Ravanat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,8,26]]},"reference":[{"key":"e_1_2_4_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/362709a0"},{"key":"e_1_2_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(91)90157-J"},{"key":"e_1_2_4_4_2","first-page":"1","volume":"31","author":"Cadet J","year":"1997","journal-title":"Rev. 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