{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T13:38:22Z","timestamp":1768743502022,"version":"3.49.0"},"reference-count":17,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,11,30]],"date-time":"2004-11-30T00:00:00Z","timestamp":1101772800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2005,1,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Previous studies of the fractionated venom of the Brazilian armed spider\n                    <jats:italic>Phoneutria nigriventer<\/jats:italic>\n                    , obtained by gel filtration, have demonstrated the presence of a fraction PhM, a pool of small peptides (up to 2000\u2009Da) that provoke contractions in smooth muscle of guinea pig ileum. Initial attempts to sequence these peptides were largely unsuccessful because of the low purification yield and the fact that the majority seemed to be blocked at their N\u2010termini. In the present work, analysis of this venom fraction by mass spectrometry has revealed the existence of a highly complex mixture of peptides with molecular weights corresponding to those observed for the muscle\u2010active peptides previously described (800\u20131800\u2009Da). These peptides appear to be a family of isoforms with some particular features. The amino acid sequences of 15 isoforms have been determined by tandem mass spectrometry (MS\/MS) using both electrospray ionization quadrupole time\u2010of\u2010flight mass spectrometry (ESI\u2010Q\/ToFMS) and matrix\u2010assisted laser desorption\/ionization tandem time\u2010of\u2010flight mass spectrometry (MALDI\u2010ToF\/ToFMS). These molecules contain post\u2010translational modifications such as proteolysis and C\u2010terminal amidation, which combine to generate additional isoforms. All the isoforms sequenced in this study possess an N\u2010terminal pyroglutamic acid residue. A search for sequence similarities with other peptides in databanks revealed that these peptides are structurally related to the tachykinins, a family of neuro\u2010hormone peptides. The data obtained in this study will be essential for the subsequent steps of this research, the synthesis of these peptides and pharmacological characterization of their biological activity. Copyright \u00a9 2004 John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/rcm.1751","type":"journal-article","created":{"date-parts":[[2004,11,30]],"date-time":"2004-11-30T08:18:45Z","timestamp":1101802725000},"page":"31-37","source":"Crossref","is-referenced-by-count":53,"title":["Electrospray ionization quadrupole time\u2010of\u2010flight and matrix\u2010assisted laser desorption\/ionization tandem time\u2010of\u2010flight mass spectrometric analyses to solve micro\u2010heterogeneity in post\u2010translationally modified peptides from\n                    <i>Phoneutria nigriventer<\/i>\n                    (Aranea, Ctenidae) venom"],"prefix":"10.1002","volume":"19","author":[{"given":"Adriano M. C.","family":"Pimenta","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Breno","family":"Rates","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"Carlos","family":"Bloch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulo C.","family":"Gomes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcelo M.","family":"Santoro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Elena","family":"de Lima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Richardson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta do N.","family":"Cordeiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,11,30]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(88)90317-0"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(90)81386-3"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(91)90195-W"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(92)81318-G"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.3109\/15569549509019466"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(94)00130-Z"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1910(01)00143-3"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2003.10.009"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M310838200"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(92)80445-M"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(03)00082-2"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.8.2444"},{"key":"e_1_2_5_14_2","unstructured":"Available:http:\/\/www.ebi.ac.uk\/fasta33\/."},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.1.138"},{"key":"e_1_2_5_16_2","unstructured":"Available:http:\/\/ca.expasy.org\/sprot\/."},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0024-3205(01)01234-6"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(03)01604-2"}],"container-title":["Rapid Communications in Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Frcm.1751","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rcm.1751","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T13:27:25Z","timestamp":1761485245000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/rcm.1751"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,11,30]]},"references-count":17,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2005,1,15]]}},"alternative-id":["10.1002\/rcm.1751"],"URL":"https:\/\/doi.org\/10.1002\/rcm.1751","archive":["Portico"],"relation":{},"ISSN":["0951-4198","1097-0231"],"issn-type":[{"value":"0951-4198","type":"print"},{"value":"1097-0231","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,11,30]]}}}