{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T20:20:17Z","timestamp":1791231617053,"version":"4.1.0"},"reference-count":25,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2006,3,7]],"date-time":"2006-03-07T00:00:00Z","timestamp":1141689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2006,4,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    A combined self\u2010aspirating electrospray emitter\/surfacing\u2010sampling probe coupled with an ion trap mass spectrometer was used to sample and mass analyze proteins from surfaces. The sampling probe mass spectrometer system was used to sample and detect lysozyme that had been deposited onto a glass slide using a piezoelectric spotter or murine gamma\u2010interferon affinity captured on a glass slide using surface\u2010immobilized anti\u2010gamma\u2010interferon antibody. The detection level for surface\u2010deposited lysozyme (spot size \u2264200\u2009\u00b5m) was approximately 1.0\u2009fmol (\u223c100\u2009fmol\/mm\n                    <jats:sup>2<\/jats:sup>\n                    ) as determined from the ability to measure accurately the protein molecular mass from the mass spectrum acquired by sampling the deposit. These detection limits may be sufficient for certain applications in which protein fractions from a separation method are collected onto a surface. Radiolabeled proteins were used to quantify the surface density of immobilized antibody and the efficiency of capture of the gamma\u2010interferon on glass and higher surface area ceramic supports. The capture density of gamma\u2010interferon at surface saturation ranged from about 23 to 50\u2009fmol\/mm\n                    <jats:sup>2<\/jats:sup>\n                    depending on the capture surface. Nonetheless, mass spectrometric detection of affinity capture protein was successful in some cases, but the results were not reproducible. Thus, improvement of the sampling system, ionization efficiency and\/or capture density will be necessary for practical sampling of affinity\u2010captured proteins. The means to accomplish improved sampling system detection limits and to increase the absolute amounts of protein captured per unit area are discussed. Published in 2006 by John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/rcm.2428","type":"journal-article","created":{"date-parts":[[2006,3,7]],"date-time":"2006-03-07T16:43:58Z","timestamp":1141749838000},"page":"1144-1152","source":"Crossref","is-referenced-by-count":31,"title":["Evaluation of a surface\u2010sampling probe electrospray mass spectrometry system for the analysis of surface\u2010deposited and affinity\u2010captured proteins"],"prefix":"10.1002","volume":"20","author":[{"given":"Gary J.","family":"Van Berkel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Ford","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitchel J.","family":"Doktycz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen J.","family":"Kennel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,3,7]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002160051563"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/1615-9861(200204)2:4<360::AID-PROT360>3.0.CO;2-Y"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac041493j"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/mas.10066"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijms.2004.10.013"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr034031f"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.R500006-MCP200"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac0415657"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-005-5006-6"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biophys.33.111502.082538"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac0482054"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1104404"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac000935y"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac020501y"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac020540"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1486"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/jms.796"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac050488s"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac0510742"},{"key":"e_1_2_6_21_2","first-page":"692","volume":"59","author":"Kennel SJ","year":"1988","journal-title":"Lab. 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