{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T09:16:48Z","timestamp":1791191808153,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2006,3,20]],"date-time":"2006-03-20T00:00:00Z","timestamp":1142812800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2006,4,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    A liquid chromatography\/electrospray ionization tandem mass spectrometry (LC\/ESI\u2010MS\/MS) method has been developed for the analysis of buprenorphine (BUP) and\n                    <jats:italic>nor<\/jats:italic>\n                    \u2010buprenorphine (NBUP) in biological fluids. Analytes are isolated from urine and blood, after addition of d\n                    <jats:sub>4<\/jats:sub>\n                    \u2010buprenorphine (d\n                    <jats:sub>4<\/jats:sub>\n                    \u2010BUP) as internal standard, by solid\u2010phase extraction. Preparation of hair involves external decontamination, mechanical pulverization, overnight incubation in acidic medium, and neutralization prior to extraction. Enzymatic hydrolysis with\n                    <jats:italic>\u03b2<\/jats:italic>\n                    \u2010glucuronidase may be performed to distinguish between free and total BUP. Chromatographic separation is accomplished by gradient elution on a cyanopropyl 2.1\u2009\u00d7\u2009150\u2009mm column. Positive ion ESI and MS analyses are carried out in an ion trap mass spectrometer. The use of this mass analyzer allows effective collisional experiments to be performed on ESI\u2010generated MH\n                    <jats:sup>+<\/jats:sup>\n                    species. Abundant product ions are produced, which can be monitored together with precursor ions without losing sensitivity. Thus, assay selectivity is definitely increased with respect to LC\/ESI\u2010MS\/MS methods in which only precursor ions are monitored. The method has good linearity (calibration curves were linear in the range 0.1\u201310\u2009ng\/mL in urine and blood, in the range 10\u2013160\u2009pg\/mg in hair) and limits of detection of 0.05\u2009ng\/mL for both BUP and NBUP in blood and urine samples, of 4\u2009pg\/mg for both analytes in hair. Both intra\u2010 and inter\u2010assay precision and accuracy were satisfactory at three concentrations studied: relative standard deviations were &lt;13.7% in urine, &lt;17.3% in blood, &lt;17.8% in hair; percent deviation of the mean from the true value was always &lt;10.5% in urine and blood, &lt;16.1% in hair. The method can be used to determine both analytes in the urine and hair of drug addicts on replacement therapy, and in post\u2010mortem blood specimens when there is suspicion of drug\u2010related death. Copyright \u00a9 2006 John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/rcm.2444","type":"journal-article","created":{"date-parts":[[2006,3,20]],"date-time":"2006-03-20T08:47:11Z","timestamp":1142844431000},"page":"1257-1265","source":"Crossref","is-referenced-by-count":33,"title":["Potentials of ion trap collisional spectrometry for liquid chromatography\/electrospray ionization tandem mass spectrometry determination of buprenorphine and\n                    <i>nor<\/i>\n                    \u2010buprenorphine in urine, blood and hair samples"],"prefix":"10.1002","volume":"20","author":[{"given":"Donata","family":"Favretto","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giampietro","family":"Frison","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanna","family":"Vogliardi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Santo Davide","family":"Ferrara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,3,20]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0029-5493(95)01058-P"},{"key":"e_1_2_5_3_2","first-page":"295","volume-title":"Opiates and Endogenous Opioid Peptides","author":"Hambrook JM","year":"1976"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002130051096"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/bja\/50.6.605"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3959(87)90042-X"},{"key":"e_1_2_5_7_2","first-page":"359","volume":"71","author":"Payne KA","year":"1987","journal-title":"Afr. 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