{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T10:06:06Z","timestamp":1761473166047,"version":"build-2065373602"},"reference-count":19,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2001,4,2]],"date-time":"2001-04-02T00:00:00Z","timestamp":986169600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2001,4,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    A strategy for the sensitive and reliable quantitative determination of non\u2010polar neutral compounds in biological matrices by liquid chromatography\/electrospray ionization tandem mass spectrometry is described in the context of assay development for TS\u2010962, a novel acyl\u2010CoA:cholesterol acyltransferase (ACAT) inhibitor, in rabbit aorta and liver tissues. The electrospray ionization (ESI) mass spectrum of this compound with a mobile phase of water\/acetonitrile did not give abundant [M\u2005+\u2005H]\n                    <jats:sup>+<\/jats:sup>\n                    ions, but did give alkali metal cation adducts such as [M\u2005+\u2005Na]\n                    <jats:sup>+<\/jats:sup>\n                    , [M\u2005+\u2005CH\n                    <jats:sub>3<\/jats:sub>\n                    CN\u2005+\u2005Na]\n                    <jats:sup>+<\/jats:sup>\n                    and [M\u2005+\u2005K]\n                    <jats:sup>+<\/jats:sup>\n                    ions. The cationized species are acknowledged as unsuitable precursor ions for selected reaction monitoring (SRM) for various reasons, such as difficulty in obtaining characteristic product ions in low\u2010energy collision\u2010induced dissociation, and irreproducibility of the adduct\u2010ion intensities. To overcome this problem, a solution of 3.4\u2005mM trifluoroacetic acid in 2\u2010propanol was added to the mobile phase as a postcolumn additive, resulting in a decrease of the undesirable adduct formation and significant enhancement of [M\u2005+\u2005H]\n                    <jats:sup>+<\/jats:sup>\n                    ion intensity. An attempt was then made to prevent the matrix effect by employing a column\u2010switching system, which allowed direct injection of a large volume of 2\u2010propanolic tissue homogenate (950\u2005\u00b5L) followed by sufficient clean\u2010up, separation, and ESI\u2010SRM on\u2010line. This enabled development of a sensitive and reliable assay method for TS\u2010962 in rabbit aorta and liver tissues in the concentration range of 5\u2013500\u2005ng\/g wet tissue using a 25\u2010mg aliquot of tissue sample. Application of this method to the determination of aortic TS\u2010962 levels at 24\u2005h after repeated oral administration of this compound (3\u2005mg\/kg) once a day for 12 weeks to 1% cholesterol\u2010fed rabbits is also presented. Results showed that TS\u2010962 is well distributed to both the thoracic and abdominal aorta tissues, at levels higher than the 50% inhibitory concentration value of this compound for microsomal ACAT activity from rabbit aorta. Copyright \u00a9 2001 John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/rcm.274","type":"journal-article","created":{"date-parts":[[2002,9,10]],"date-time":"2002-09-10T16:17:06Z","timestamp":1031674626000},"page":"629-636","source":"Crossref","is-referenced-by-count":5,"title":["A strategy for quantitative bioanalysis of non\u2010polar neutral compounds by liquid chromatography\/electrospray ionization tandem mass spectrometry: determination of TS\u2010962, a novel acyl\u2010CoA:cholesterol acyltransferase inhibitor, in rabbit aorta and liver tissues"],"prefix":"10.1002","volume":"15","author":[{"given":"Jun\u2010ichi","family":"Yamaguchi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yumiko","family":"Matsuno","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenji","family":"Hachiuma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoyoshi","family":"Ogawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shohei","family":"Higuchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,4,2]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac00212a002"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/js9701059"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac990433o"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0231(19970630)11:10<1083::AID-RCM937>3.0.CO;2-F"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.5702\/massspec.47.229"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1044-0305(99)00088-4"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1044-0305(96)00072-4"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac971078"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)34322-4"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)70568-7"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0024-3205(98)00028-9"},{"key":"e_1_2_6_13_2","unstructured":"AsamiY TomoikeH AkiyoshiK YamagishiI OgawaN TsuchidaK HiguchiS. 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