{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T13:34:30Z","timestamp":1761485670159,"version":"build-2065373602"},"reference-count":27,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2001,4,11]],"date-time":"2001-04-11T00:00:00Z","timestamp":986947200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2001,5,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>A sensitive and robust liquid chromatography\/electrospray ion trap mass spectrometry (LC\/MS\/MS) method has been developed for the quantitative determination of noncovalently bound acridinium free acid in protein\u2010acridinium conjugates. The lower level of quantitation (LOQ) for acridinium free acid was determined to be 0.6\u2005ng. The assay was validated with a linear concentration range of 0.6\u201360\u2005ng. The method requires minimum sample handling and is specific, reproducible, and provides a new aspect for protein\u2010acridinium conjugate characterization. Copyright \u00a9 2001 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/rcm.282","type":"journal-article","created":{"date-parts":[[2002,9,10]],"date-time":"2002-09-10T16:17:06Z","timestamp":1031674626000},"page":"670-674","source":"Crossref","is-referenced-by-count":2,"title":["Quantitative determination of noncovalently bound acridinium in protein conjugates by liquid chromatography\/electrospray ion trap mass spectrometry"],"prefix":"10.1002","volume":"15","author":[{"given":"Maciej","family":"Adamczyk","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John C.","family":"Gebler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin","family":"Shreder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiang","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,4,11]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-012342335-1\/50010-5"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac962124t"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1021\/bc00030a019"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/bc980029g"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/bc960080p"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(94)90081-7"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(79)90792-9"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac00021a016"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/bc960035h"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/bc950064c"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1385\/0-89603-345-7:211"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac970035q"},{"key":"e_1_2_5_14_2","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1093\/labmed\/29.9.555","volume":"29","author":"Jandreski MA","year":"1998","journal-title":"Lab. Med."},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1177\/000456328802500103"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/jo980047r"},{"key":"e_1_2_5_17_2","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1093\/clinchem\/32.3.532","volume":"32","author":"Sturgess ML","year":"1986","journal-title":"Clin. Chem."},{"journal-title":"Bioconjugate Chem.","year":"2000","author":"Adamczyk M","key":"e_1_2_5_18_2"},{"key":"e_1_2_5_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(98)00003-6"},{"key":"e_1_2_5_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(98)00078-8"},{"key":"e_1_2_5_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(98)00281-7"},{"key":"e_1_2_5_22_2","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/S0378-4347(97)00653-1","volume":"708","author":"Wieboldt R","year":"1998","journal-title":"J. Chromatogr. B"},{"key":"e_1_2_5_23_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac9813540"},{"key":"e_1_2_5_24_2","first-page":"444","volume-title":"Introduction to Mass Spectrometry","author":"Watson JT","year":"1997"},{"key":"e_1_2_5_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-894X(98)00656-8"},{"key":"e_1_2_5_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-4020(99)00624-9"},{"key":"e_1_2_5_27_2","first-page":"37","volume-title":"Bioluminescence and Chemiluminescence. Perspectives for the 21st Century","author":"Mattingly PG","year":"1999"},{"key":"e_1_2_5_28_2","doi-asserted-by":"publisher","DOI":"10.1021\/bc9700392"}],"container-title":["Rapid Communications in Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Frcm.282","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rcm.282","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T13:28:55Z","timestamp":1761485335000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/rcm.282"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,4,11]]},"references-count":27,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2001,5,15]]}},"alternative-id":["10.1002\/rcm.282"],"URL":"https:\/\/doi.org\/10.1002\/rcm.282","archive":["Portico"],"relation":{},"ISSN":["0951-4198","1097-0231"],"issn-type":[{"type":"print","value":"0951-4198"},{"type":"electronic","value":"1097-0231"}],"subject":[],"published":{"date-parts":[[2001,4,11]]}}}