{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T07:39:26Z","timestamp":1792136366997,"version":"4.4.0"},"reference-count":23,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2007,2,22]],"date-time":"2007-02-22T00:00:00Z","timestamp":1172102400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2007,3,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Variation in the snake venom proteome is well documented and it is a ubiquitous phenomenon at all taxonomical levels. However, variation in the snake venom peptidome is so far not described. In this work we used mass spectrometry [liquid chromatography\/mass spectrometry (LC\/MS) and matrix\u2010assisted laser desorption\/ionization time\u2010of\u2010flight (MALDI\u2010TOFMS)] to explore sex\u2010based differences among the venom peptides of eighteen sibling specimens of\n                    <jats:italic>Bothrops jararaca<\/jats:italic>\n                    of a single litter born and raised in the laboratory. MALDI\u2010TOFMS analyses showed individual variability among the bradykinin\u2010potentiating peptides (BPPs), and, interestingly, four new peptides were detected only in female venoms and identified by\n                    <jats:italic>de novo<\/jats:italic>\n                    sequencing as cleaved BPPs lacking the C\u2010terminal Q\u2010I\u2010P\u2010P sequence. Similar results were obtained with venom from wild\u2010caught adult non\u2010sibling specimens of\n                    <jats:italic>B. jararaca<\/jats:italic>\n                    and in this case we were able to identify the gender of the specimen by analyzing the MALDI\u2010TOF profile of the peptide fraction and finding the cleaved peptides only in female venoms. Synthetic replicates of the cleaved BPPs were less potent than the full\u2010length BPP\u201010c in potentiating the bradykinin hypotensive effect, suggesting that the C\u2010terminus is critical for the interaction of the BPPs with their mammalian molecular targets. This work represents a comprehensive mass spectrometric analysis of the peptide fraction of\n                    <jats:italic>B. jararaca<\/jats:italic>\n                    venom and shows for the first time sex\u2010based differences in the snake venom peptidome of sibling and non\u2010sibling snakes and suggests that the BPPs may follow distinct processing pathways in female and male individuals. Copyright \u00a9 2007 John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/rcm.2931","type":"journal-article","created":{"date-parts":[[2007,2,22]],"date-time":"2007-02-22T07:12:52Z","timestamp":1172128372000},"page":"1034-1042","source":"Crossref","is-referenced-by-count":84,"title":["Mass spectrometric analysis of the individual variability of\n                    <i>Bothrops jararaca<\/i>\n                    venom peptide fraction. Evidence for sex\u2010based variation among the bradykinin\u2010potentiating peptides"],"prefix":"10.1002","volume":"21","author":[{"given":"Daniel C.","family":"Pimenta","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benedito C.","family":"Prezoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsuhiro","family":"Konno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robson L.","family":"Melo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria F.","family":"Furtado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio C. M.","family":"Camargo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Solange M. T.","family":"Serrano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,2,22]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-66913-2"},{"key":"e_1_2_7_3_2","first-page":"97","volume-title":"Perspectives in Molecular Toxinology","author":"Fox JW","year":"2002"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200400931"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2006.01.022"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0020-711X(78)90001-0"},{"key":"e_1_2_7_7_2","first-page":"163","volume":"34","author":"Ferreira SH","year":"1965","journal-title":"Br. J. 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