{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T19:00:17Z","timestamp":1782586817486,"version":"3.54.5"},"reference-count":38,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T00:00:00Z","timestamp":1172620800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2007,4,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The roots and leaves of\n                    <jats:italic>Isatis indigotica<\/jats:italic>\n                    , named \u2018Ban\u2010Lan\u2010Gen\u2019 and \u2018Da\u2010Qing\u2010Ye\u2019, respectively, are widely used for the treatment of influenza, viral pneumonia, mumps, pharyngitis, and hepatitis. The indoxyl derivatives detected in the roots and leaves of\n                    <jats:italic>I. indigotica<\/jats:italic>\n                    have been reported to be biologically active. In the present study, a liquid chromatography\/electrospray ionization tandem mass spectrometry (LC\/ESI\u2010MS\/MS) method was developed to determine indican, isatin, indirubin and indigotin in the roots and leaves of\n                    <jats:italic>I. indigotica<\/jats:italic>\n                    . The method has been validated for linearity, precision and accuracy. Using multiple reaction monitoring (MRM), the limits of detection (LODs) were determined as 0.004\u2009ng for indican, 0.01\u2009ng for isatin, 0.01\u2009ng for indrubin and 0.03\u2009ng for indigotin, while the limits of quantitation (LOQs) were 0.015\u2009ng for indican, 0.04\u2009ng for isatin, 0.04\u2009ng for indirubin and 0.1\u2009ng for indigotin. Compared with previously reported methods, the current method is more rapid, selective and sensitive. This is the first report of the LC\/MS\/MS determination of indican, isatin, indirubin and indigotin. Copyright \u00a9 2007 John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/rcm.2954","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T08:54:55Z","timestamp":1172652895000},"page":"1239-1246","source":"Crossref","is-referenced-by-count":57,"title":["Determination of indican, isatin, indirubin and indigotin in\n                    <i>Isatis indigotica<\/i>\n                    by liquid chromatography\/electrospray ionization tandem mass spectrometry"],"prefix":"10.1002","volume":"21","author":[{"given":"Peng","family":"Zou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hwee Ling","family":"Koh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,2,28]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"177","volume-title":"Chinese Pharmacopoeia (English Version)","author":"Ministry of Public Health","year":"2000"},{"key":"e_1_2_6_3_2","first-page":"a423","volume":"17","author":"Chang YS","year":"2001","journal-title":"Anal. 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