{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T08:26:10Z","timestamp":1771662370538,"version":"3.50.1"},"reference-count":11,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2007,5,8]],"date-time":"2007-05-08T00:00:00Z","timestamp":1178582400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2007,6,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Tiotropium bromide, a long\u2010acting inhaled bronchodilator analogous to ipratropium bromide, is currently undergoing development for the treatment of chronic obstructive pulmonary disease. To evaluate its systemic absorption in humans, we have developed a rapid and sensitive method for its determination in human plasma based on high\u2010performance liquid chromatography with tandem mass spectrometric detection (HPLC\/MS\/MS). Reversed\u2010phase chromatography of tiotropium and the internal standard clenbuterol was carried out using acetonitrile\/10\u2009mM ammonium acetate (1% formic acid) 40:60 as mobile phase in a run time of 3.0\u2009min. The sample preparation involved deproteination with acetonitrile, extraction into dichloromethane and back\u2010extraction into hydrochloric acid. The assay was linear over the concentration range 0.500\u201350.0\u2009pg\/mL with intra\u2010 and inter\u2010day precision (as relative standard deviation) both \u22647.34%. The method was successfully applied to a pharmacokinetic study of systemic absorption in healthy male volunteers given a single 18\u2009\u00b5g inhaled dose. Copyright \u00a9 2007 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/rcm.3021","type":"journal-article","created":{"date-parts":[[2007,5,8]],"date-time":"2007-05-08T06:47:10Z","timestamp":1178606830000},"page":"1755-1758","source":"Crossref","is-referenced-by-count":22,"title":["Highly sensitive assay for tiotropium, a quaternary ammonium, in human plasma by high\u2010performance liquid chromatography\/tandem mass spectrometry"],"prefix":"10.1002","volume":"21","author":[{"given":"Jiang","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yao","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingwu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. Paul","family":"Fawcett","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingkai","family":"Gu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,5,8]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinthera.2005.04.006"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(95)00020-7"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1517\/13543784.10.4.733"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-200262080-00008"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1358\/dot.2002.38.9.696535"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4892(03)00047-X"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pupt.2005.02.013"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1177\/0091270003261315"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/bdd.280"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0024-3205(98)00588-8"},{"key":"e_1_2_5_12_2","unstructured":"FDA.Guidance for Industry Bioanalytical Method Validation May 2001."}],"container-title":["Rapid Communications in Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Frcm.3021","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rcm.3021","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T11:21:27Z","timestamp":1761477687000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/rcm.3021"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,5,8]]},"references-count":11,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2007,6,15]]}},"alternative-id":["10.1002\/rcm.3021"],"URL":"https:\/\/doi.org\/10.1002\/rcm.3021","archive":["Portico"],"relation":{},"ISSN":["0951-4198","1097-0231"],"issn-type":[{"value":"0951-4198","type":"print"},{"value":"1097-0231","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,5,8]]}}}