{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T21:01:05Z","timestamp":1761512465818,"version":"build-2065373602"},"reference-count":21,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2007,10,30]],"date-time":"2007-10-30T00:00:00Z","timestamp":1193702400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2007,12,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    We describe a convenient and useful method for the identification and relative quantification of proteins using light and heavy reagents, 1\u2010(6\u2010methylnicotinoyloxy)succinimides (6\u2010CH\n                    <jats:sub>3<\/jats:sub>\n                    \u2010Nic\u2010NHS and 6\u2010CD\n                    <jats:sub>3<\/jats:sub>\n                    \u2010Nic\u2010NHS, respectively). This method is based on the chemical derivatization of amino groups of tryptic peptides with these reagents, i.e., the basic moiety of the reagents thus incorporated into both the N\u2010terminal amino group and the\n                    <jats:italic>\u03b5<\/jats:italic>\n                    \u2010amino group of the lysine residue would improve the ionization efficiency of tryptic peptides. An increase in protein sequence coverage is achieved by derivatization with these reagents or by combination of mass values before and after derivatization. Since a combination of 6\u2010CH\n                    <jats:sub>3<\/jats:sub>\n                    \u2010Nic\u2010NHS and d\n                    <jats:sub>3<\/jats:sub>\n                    \u2010labeled reagent (6\u2010CD\n                    <jats:sub>3<\/jats:sub>\n                    \u2010Nic\u2010NHS) generates a 3\u2009Da mass difference per reaction site, the d\n                    <jats:sub>3<\/jats:sub>\n                    \u2010labeled reagent shifts the mass values of d\n                    <jats:sub>0<\/jats:sub>\n                    \u2010labeled peptides according to the number of reactive amino groups in the peptides. In the case of tryptic peptides, the mass values of C\u2010terminal arginine and lysine peptides are shifted by 3 and 6\u2009Da, respectively. Further, the 3\u2009Da mass difference between 6\u2010CH\n                    <jats:sub>3<\/jats:sub>\n                    \u2010Nic\u2010NHS and 6\u2010CD\n                    <jats:sub>3<\/jats:sub>\n                    \u2010Nic\u2010NHS offers a means for the relative quantification of protein by matrix\u2010assisted laser desorption\/ionization time\u2010of\u2010flight mass spectrometry. Copyright \u00a9 2007 John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/rcm.3279","type":"journal-article","created":{"date-parts":[[2007,10,30]],"date-time":"2007-10-30T14:38:39Z","timestamp":1193755119000},"page":"3815-3824","source":"Crossref","is-referenced-by-count":9,"title":["Efficient identification and quantification of proteins using isotope\u2010coded 1\u2010(6\u2010methylnicotinoyloxy)succinimides by matrix\u2010assisted laser desorption\/ionization time\u2010of\u2010flight mass spectrometry"],"prefix":"10.1002","volume":"21","author":[{"given":"Hiroki","family":"Tsumoto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chie","family":"Murata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoki","family":"Miyata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kohfuku","family":"Kohda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryo","family":"Taguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,11,27]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.2166"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac990298f"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.2000.4834"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0231(20001215)14:23<2147::AID-RCM145>3.0.CO;2-M"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac015613o"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0202-163"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac030421"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr0255506"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac050540k"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2005.11.015"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio736"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M200025-MCP200"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200400873"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/13690"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2006.09.023"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac000265w"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M400129-MCP200"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1100"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.362"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac001404c"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1248\/cpb.51.1399"}],"container-title":["Rapid Communications in Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Frcm.3279","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rcm.3279","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T11:17:52Z","timestamp":1761477472000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/rcm.3279"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,11,27]]},"references-count":21,"journal-issue":{"issue":"23","published-print":{"date-parts":[[2007,12,15]]}},"alternative-id":["10.1002\/rcm.3279"],"URL":"https:\/\/doi.org\/10.1002\/rcm.3279","archive":["Portico"],"relation":{},"ISSN":["0951-4198","1097-0231"],"issn-type":[{"type":"print","value":"0951-4198"},{"type":"electronic","value":"1097-0231"}],"subject":[],"published":{"date-parts":[[2007,11,27]]}}}