{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T07:17:45Z","timestamp":1792135065153,"version":"4.4.0"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2008,1,10]],"date-time":"2008-01-10T00:00:00Z","timestamp":1199923200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2008,2,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The analysis of peptide mixtures from urine and plasma samples using bare (uncapped) SiO\n                    <jats:sub>2<\/jats:sub>\n                    nanoparticles (NPs) with atmospheric\u2010pressure matrix\u2010assisted laser desorption\/ionization mass spectrometry (AP\u2010MALDI\u2010MS) has been reported. The method was based on the adsorption of positively charged peptides on the surface of negatively charged SiO\n                    <jats:sub>2<\/jats:sub>\n                    NPs through the electrostatic force of attraction. The adsorption on the surface of SiO\n                    <jats:sub>2<\/jats:sub>\n                    NPs caused enhancement of ionization efficiency of analytes and subsequently increased the signal intensity of peptides. Maximum signal intensity was obtained at optimized concentration of SiO\n                    <jats:sub>2<\/jats:sub>\n                    NPs and pH of the aqueous solution. The limits of detection (LODs) obtained for different peptides in deionized water with and without using SiO\n                    <jats:sub>2<\/jats:sub>\n                    NPs were in the range 4.7\u2013360\u2009nM and 0.1\u201318.0\u2009\u00b5M, respectively. The sensitivity of the proposed method was 21\u201353\u2010fold better than conventional use of AP\u2010MALDI\u2010MS. In addition, linearity in the range 9.5\u201395\u2009nM was obtained for the peptide angiotensin\u2010II in deionized water with a correlation of estimation of 0.992 using an internal standard. The proposed method provided a simple way to facilitate the ionization of peptides, reduce sample complexity and increase the tolerance to salts and surfactants in the analysis of biological samples. The applicability of the present method was also demonstrated in the real\u2010world sample analysis of aminothiols and lysozyme using MALDI\u2010time\u2010of\u2010flight (TOF)\u2010MS. Copyright \u00a9 2008 John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/rcm.3363","type":"journal-article","created":{"date-parts":[[2008,1,10]],"date-time":"2008-01-10T11:46:58Z","timestamp":1199965618000},"page":"283-290","source":"Crossref","is-referenced-by-count":46,"title":["Bare silica nanoparticles as concentrating and affinity probes for rapid analysis of aminothiols, lysozyme and peptide mixtures using atmospheric\u2010pressure matrix\u2010assisted laser desorption\/ionization ion trap and matrix\u2010assisted laser desorption\/ionization time\u2010of\u2010flight mass spectrometry"],"prefix":"10.1002","volume":"22","author":[{"given":"Kavita","family":"Agrawal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui\u2010Fen","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,1,10]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac00171a028"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr0604460"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac025907p"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac015550"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac048391d"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf9809380"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac980684h"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac0604181"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac051162m"},{"key":"e_1_2_6_11_2"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1634"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac048971a"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac0517646"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac070847e"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac061747w"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac050831t"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac061154l"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-006-0427-0"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja973849x"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2744(70)90171-3"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2006.03.008"},{"key":"e_1_2_6_23_2","first-page":"44","volume":"2","author":"Yan J","year":"2007","journal-title":"Review"},{"key":"e_1_2_6_24_2"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/jms.1261"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja0358854"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2006.09.055"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.susc.2006.09.019"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac060123n"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1051"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac000530d"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac990998k"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1143\/JJAP.45.4259"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1290070402"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.2736"},{"key":"e_1_2_6_36_2","author":"Kusmierek K","year":"2006","journal-title":"Anal. 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