{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T15:18:26Z","timestamp":1764688706588,"version":"build-2065373602"},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2008,12,8]],"date-time":"2008-12-08T00:00:00Z","timestamp":1228694400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2009,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Sensitive and reliable methods are required for the assessment of oxidative DNA damage, which can result from reactive oxygen species that are generated endogenously from cellular metabolism and inflammatory responses, or by exposure to exogenous agents. The development of a liquid chromatography\/tandem mass spectrometry (LC\/MS\/MS) selected reaction monitoring (SRM) method is described, that utilises online column\u2010switching valve technology for the simultaneous determination of two DNA adduct biomarkers of oxidative stress, 8\u2010oxo\u20107,8\u2010dihydro\u20102\u2032\u2010deoxyguanosine (8\u2010oxodG) and 8\u2010oxo\u20107,8\u2010dihydro\u20102\u2032\u2010deoxyadenosine (8\u2010oxodA). To allow for the accurate quantitation of both adducts the corresponding [\n                    <jats:sup>15<\/jats:sup>\n                    N\n                    <jats:sub>5<\/jats:sub>\n                    ]\u2010labelled stable isotope internal standards were synthesised and added prior to enzymatic hydrolysis of the DNA samples to 2\u2032\u2010deoxynucleosides. The method required between 10 and 40\u2009\u00b5g of hydrolysed DNA on\u2010column for the analysis and the limit of detection for both 8\u2010oxodG and 8\u2010oxodA was 5\u2009fmol. The analysis of calf thymus DNA treated\n                    <jats:italic>in vitro<\/jats:italic>\n                    with methylene blue (ranging from 5 to 200\u2009\u00b5M) plus light showed a dose\u2010dependent increase in the levels of both 8\u2010oxodG and 8\u2010oxodA. The level of 8\u2010oxodG was on average 29.4\u2010fold higher than that of 8\u2010oxodA and an excellent linear correlation (r\u2009=\u20090.999) was observed between the two adducts. The influence of different DNA extraction procedures for 8\u2010oxodG and 8\u2010oxodA levels was assessed in DNA extracted from rat livers following dosing with carbon tetrachloride. The levels of 8\u2010oxodG and 8\u2010oxodA were on average 2.9 (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.018) and 1.4 (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.018) times higher, respectively, in DNA samples extracted using an anion\u2010exchange column procedure than in samples extracted using a chaotropic procedure, implying artefactual generation of the two adducts. In conclusion, the online column\u2010switching LC\/MS\/MS SRM method provides the advantages of increased sample throughput with reduced matrix effects and concomitant ionisation suppression, making the method ideally suited when used in conjunction with chaotropic DNA extraction for the determination of oxidative DNA damage. Copyright \u00a9 2008 John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/rcm.3866","type":"journal-article","created":{"date-parts":[[2008,12,8]],"date-time":"2008-12-08T04:13:39Z","timestamp":1228709619000},"page":"151-160","source":"Crossref","is-referenced-by-count":36,"title":["Simultaneous determination of 8\u2010oxo\u20102\u2032\u2010deoxyguanosine and 8\u2010oxo\u20102\u2032\u2010deoxyadenosine in DNA using online column\u2010switching liquid chromatography\/tandem mass spectrometry"],"prefix":"10.1002","volume":"23","author":[{"given":"Rajinder","family":"Singh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Friederike","family":"Teichert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard 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