{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T00:27:18Z","timestamp":1761611238610,"version":"build-2065373602"},"reference-count":26,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2009,1,5]],"date-time":"2009-01-05T00:00:00Z","timestamp":1231113600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2009,2,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    A technique using comprehensive two\u2010dimensional gas chromatography\/time\u2010of\u2010flight mass spectrometry (GC\u2009\u00d7\u2009GC\/TOFMS) is applied to qualitative and quantitative drug testing. Human serum was \u2018spiked\u2019 with known quantities of benzodiazepines and a \u2018street heroin\u2019 mixture including some of the major metabolites and impurities. The sample components were extracted from the matrix by solid\u2010phase extraction (SPE). Constituents containing polar hydroxyl and\/or secondary amine groups were derivatised with\n                    <jats:italic>N<\/jats:italic>\n                    \u2010methyl\u2010\n                    <jats:italic>N<\/jats:italic>\n                    \u2010(\n                    <jats:italic>tert<\/jats:italic>\n                    \u2010butyldimethyl)trifluoroacetamide (MTBSTFA) to improve the chromatographic performance. An orthogonal separation of the matrix constituents was achieved by coupling a DB\u20105ms (5% phenyl) to a BPX50 (50% phenyl) GC column. The eluant was focused onto the second column by a twin\u2010stage cryo\u2010modulator. Rapid 6\u2009s modulation times were achieved by transfer from a 30\u2009m\u2009\u00d7\u20090.25\u2009mm (length\u2009\u00d7\u2009internal diameter) to a 2\u2009m\u2009\u00d7\u20090.1\u2009mm column. TOFMS with rapid spectral acquisition (\u2264500 spectra\/s) was employed in the mass range\n                    <jats:italic>m\/z<\/jats:italic>\n                    40\u2013650. A clean mass spectrum was obtained for each analyte using mass spectral deconvolution software. The sensitivity and repeatability of the method were evaluated by the preparation of calibration standards for two benzodiazepines, flunitrazepam and its major metabolite 7\u2010aminoflunitrazepam (7\u2010amino\u2010FN), in the concentration range 5\u20131000\u2009ng\/mL. The limits of detection (LODs) and limits of quantitation (LOQs), calculated by repeat injections (\u00d710) of the lowest standard, were 1.6 and 5.4\u2009ng\/mL (flunitrazepam); 2.5 and 8.5\u2009ng\/mL (7\u2010amino\u2010FN), respectively. There is scope to extend this protocol to screen a large number of drugs and metabolites stored in a library database. Copyright \u00a9 2009 John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/rcm.3883","type":"journal-article","created":{"date-parts":[[2009,1,9]],"date-time":"2009-01-09T14:30:12Z","timestamp":1231511412000},"page":"340-348","source":"Crossref","is-referenced-by-count":17,"title":["The detection of various opiates and benzodiazepines by comprehensive two\u2010dimensional gas chromatography\/time\u2010of\u2010flight mass spectrometry"],"prefix":"10.1002","volume":"23","author":[{"given":"Bill","family":"Guthery","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan","family":"Bassindale","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Colin T.","family":"Pillinger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geraint H.","family":"Morgan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,5]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1093\/clinchem\/47.2.301","volume":"47","author":"Staub C","year":"2001","journal-title":"Clin. 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