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Then, an acidic PLA\n                    <jats:sub>2<\/jats:sub>\n                    with the accurate molecular mass of 13979.6\u2009Da was purified from ABUSV (mo\u2010ABUSV\u2010aPLA\n                    <jats:sub>2<\/jats:sub>\n                    ). MS\/MS\u2010derived peptides from ABUSV\u2010aPLA\n                    <jats:sub>2<\/jats:sub>\n                    were compared with other homologous snake venom PLA\n                    <jats:sub>2<\/jats:sub>\n                    s, which in turn showed that ABUSV\u2010aPLA\n                    <jats:sub>2<\/jats:sub>\n                    is a novel snake venom PLA\n                    <jats:sub>2<\/jats:sub>\n                    . Meanwhile, the ABUSV\u2010aPLA\n                    <jats:sub>2<\/jats:sub>\n                    dimer (di\u2010ABUSV\u2010aPLA\n                    <jats:sub>2<\/jats:sub>\n                    ) was also obtained. MS\/MS analysis identified the same peptides from di\u2010ABUSV\u2010aPLA\n                    <jats:sub>2<\/jats:sub>\n                    as from mo\u2010ABUSV\u2010aPLA\n                    <jats:sub>2<\/jats:sub>\n                    , which indicates that di\u2010ABUSV\u2010aPLA\n                    <jats:sub>2<\/jats:sub>\n                    is a homodimer. One Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ion is contained per ABUSV\u2010aPLA\n                    <jats:sub>2<\/jats:sub>\n                    . The Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ion is critical for both the hydrolytic activity and the structure of ABUSV\u2010aPLA\n                    <jats:sub>2<\/jats:sub>\n                    . Pro\u2010Q Emerald and Pro\u2010Q Diamond specific glycoprotein and phosphoprotein staining combined with MS\/MS analysis indicated that the ABUSV\u2010aPLA\n                    <jats:sub>2<\/jats:sub>\n                    is both a glycoprotein and a phosphoprotein, which to our knowledge is the first such report for a snake venom PLA\n                    <jats:sub>2<\/jats:sub>\n                    and thus provides new threads for the study of the functions and structures of snake venom PLA\n                    <jats:sub>2<\/jats:sub>\n                    s. One phosphorylation site and the size of the glycan chain are determined by using HPLC\/nESI\u2010MS\/MS and matrix\u2010assisted laser desorption\/ionization time\u2010of\u2010flight (MALDI\u2010TOF) MS. The delicate utilization of ESI\u2010MS can exert tremendous impact on protein sciences. Copyright \u00a9 2009 John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/rcm.3996","type":"journal-article","created":{"date-parts":[[2009,3,12]],"date-time":"2009-03-12T10:59:15Z","timestamp":1236855555000},"page":"1158-1166","source":"Crossref","is-referenced-by-count":11,"title":["Electrospray ionization mass spectrometry as a critical tool for revealing new properties of snake venom phospholipase A\n                    <sub>2<\/sub>"],"prefix":"10.1002","volume":"23","author":[{"given":"Shuqing","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cuili","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue\u2010Fei","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming\u2010Zhong","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,3,12]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1042\/0264-6021:3460631"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.08.046"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2004.08.006"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200400931"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0231(19990215)13:3<150::AID-RCM471>3.0.CO;2-6"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jasms.2008.04.020"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr0682047"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2006.07.183"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2006.06.003"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.3483"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-55647-0_7"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.3725"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1097\/FTD.0b013e31816b92c9"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.23329"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2006.02.069"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200500921"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/1044-0305(94)80016-2"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/22.22.4673"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btm404"},{"key":"e_1_2_6_21_2","first-page":"104","volume":"31","author":"Sun M\u2010Z","year":"1999","journal-title":"Acta Biochim. 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