{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T03:17:31Z","timestamp":1777346251301,"version":"3.51.4"},"reference-count":23,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2009,8,3]],"date-time":"2009-08-03T00:00:00Z","timestamp":1249257600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2009,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Chemical cross\u2010linking combined with a subsequent enzymatic digestion and mass spectrometric analysis of the created cross\u2010linked products presents an alternative approach to assess low\u2010resolution protein structures. By covalently connecting pairs of functional groups within a protein or a protein complex a set of structurally defined interactions is built up. We synthesized the heterobifunctional amine\u2010reactive photo\u2010cross\u2010linker\n                    <jats:italic>N<\/jats:italic>\n                    \u2010succinimidyl\n                    <jats:italic>p\u2010<\/jats:italic>\n                    benzoyldihydrocinnamate as a non\u2010deuterated (SBC) and doubly deuterated derivative (SBDC). Applying a 1:1 mixture of SBC and SBDC for cross\u2010linking experiments aided the identification of cross\u2010linked amino acids in the mass spectra based on the characteristic isotope patterns of fragment ions. The cross\u2010linker was applied to the calcium\u2010binding protein calmodulin with a subsequent analysis of cross\u2010linked products by nano\u2010high\u2010performance liquid chromatography matrix\u2010assisted laser desorption\/ionization tandem time\u2010of\u2010flight mass spectrometry (nano\u2010HPLC\/MALDI\u2010TOF\/TOF\u2010MS) and nano\u2010HPLC\/nano\u2010electrospray ionization (ESI)\u2010LTQ\u2010Orbitrap\u2010MS. Copyright \u00a9 2009 John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/rcm.4188","type":"journal-article","created":{"date-parts":[[2009,8,3]],"date-time":"2009-08-03T06:06:11Z","timestamp":1249279571000},"page":"2811-2818","source":"Crossref","is-referenced-by-count":35,"title":["Heterobifunctional isotope\u2010labeled amine\u2010reactive photo\u2010cross\u2010linker for structural investigation of proteins by matrix\u2010assisted laser desorption\/ionization tandem time\u2010of\u2010flight and electrospray ionization LTQ\u2010Orbitrap mass spectrometry"],"prefix":"10.1002","volume":"23","author":[{"given":"Fabian","family":"Krauth","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian H.","family":"Ihling","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans Hermann","family":"R\u00fcttinger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Sinz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,8,3]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.090099097"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(03)00721-6"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1144"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/jms.559"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/mas.20082"},{"key":"e_1_2_6_7_2","volume-title":"Bioconjugate Techniques","author":"Hermanson GT","year":"2008"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.62.070193.002411"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3011.1997.tb00889.x"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2006.02.050"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1747-0285.2008.00646.x"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1021\/bc00034a009"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1255\/ejms.748"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jasms.2006.04.020"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00058a004"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi036149f"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-008-2231-5"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(01)01954-5"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/jms.1544"},{"key":"e_1_2_6_20_2","unstructured":"http:\/\/structbio.vanderbilt.edu\/cabp_database\/general\/prot_pages\/calmod.html"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi900300r"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1103"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2003.09.004"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1110\/ps.04846604"}],"container-title":["Rapid Communications in Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Frcm.4188","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Frcm.4188","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rcm.4188","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T11:22:17Z","timestamp":1761477737000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/rcm.4188"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,8,3]]},"references-count":23,"journal-issue":{"issue":"17","published-print":{"date-parts":[[2009,9,15]]}},"alternative-id":["10.1002\/rcm.4188"],"URL":"https:\/\/doi.org\/10.1002\/rcm.4188","archive":["Portico"],"relation":{},"ISSN":["0951-4198","1097-0231"],"issn-type":[{"value":"0951-4198","type":"print"},{"value":"1097-0231","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,8,3]]}}}