{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T22:05:55Z","timestamp":1787263555860,"version":"build-2736575974"},"reference-count":25,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2009,10,8]],"date-time":"2009-10-08T00:00:00Z","timestamp":1254960000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2009,11,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>A broadly applicable, robust, and rapid method for complete sequence confirmation of highly modified oligonucleotides containing a mixture of 2\u2032\u2010deoxy, 2\u2032\u2010fluoro, 2\u2032\u2010o\u2010methyl, abasic and ribonucleotides is presented. The passenger (sense) and guide (antisense) strands from synthetic short interfering RNA duplexes (siRNA) were digested individually using both 5\u2032\u2010 and 3\u2032\u2010exonucleases and the resulting ladders were analyzed using matrix\u2010assisted laser desorption\/ionization time\u2010of\u2010flight (MALDI\u2010TOF) mass spectrometry. Conditions for enzymatic digestion and MALDI\u2010TOF mass analysis were investigated and optimized, and the digestion pattern and sequence coverage of each strand was discussed. Complete sequence confirmation for the antisense strands of four synthetic RNA duplexes was obtained, whereas a three\u2010base sequence gap in the 5\u2032\u2010end was observed for all four sense strands. A general strategy is proposed for routine sequence confirmation of highly modified oligonucleotides, and the potential for complete automation of the method is also discussed. Copyright \u00a9 2009 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/rcm.4266","type":"journal-article","created":{"date-parts":[[2009,10,8]],"date-time":"2009-10-08T04:31:08Z","timestamp":1254976268000},"page":"3423-3430","source":"Crossref","is-referenced-by-count":15,"title":["Sequence confirmation of chemically modified RNAs using exonuclease digestion and matrix\u2010assisted laser desorption\/ionization time\u2010of\u2010flight mass spectrometry"],"prefix":"10.1002","volume":"23","author":[{"given":"Hong","family":"Gao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan","family":"Rumley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huimin","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Mao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,10,8]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00581-5"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0502-446"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrd837"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1089\/oli.1.1998.8.135"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4781(99)00148-7"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1999.17.11.3586"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(20010901)92:5<1265::AID-CNCR1447>3.0.CO;2-5"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1177\/00912709922007552"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2006.10.001"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/21.11.2709"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2004.07.021"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2006.01.004"},{"key":"e_1_2_6_14_2","first-page":"282","volume":"1075","author":"Ding CM","year":"2006","journal-title":"Circulating Nucleic Acids in Plasma and Serum IV"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1996.0243"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.2002.5641"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.3561"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jasms.2005.03.024"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1255\/ejms.736"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac960865o"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijms.2004.06.014"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jasms.2008.04.034"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2005.06.045"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1021\/tx010062i"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac8011158"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1387-3806(02)00978-8"}],"container-title":["Rapid Communications in Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Frcm.4266","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Frcm.4266","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rcm.4266","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T14:37:51Z","timestamp":1761489471000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/rcm.4266"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,10,8]]},"references-count":25,"journal-issue":{"issue":"21","published-print":{"date-parts":[[2009,11,15]]}},"alternative-id":["10.1002\/rcm.4266"],"URL":"https:\/\/doi.org\/10.1002\/rcm.4266","archive":["Portico"],"relation":{},"ISSN":["0951-4198","1097-0231"],"issn-type":[{"value":"0951-4198","type":"print"},{"value":"1097-0231","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,10,8]]}}}