{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T05:06:32Z","timestamp":1777871192306,"version":"3.51.4"},"reference-count":41,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2010,4,6]],"date-time":"2010-04-06T00:00:00Z","timestamp":1270512000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2010,5,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    We compare a new simplified\n                    <jats:sup>2<\/jats:sup>\n                    H enrichment mass isotopomer analysis (MIA) against the laborious hexamethylentetramine (HMT) method to quantify the contribution of gluconeogenesis (GNG) to total glucose production (GP) in calves. Both methods are based on the\n                    <jats:sup>2<\/jats:sup>\n                    H labeling of glucose after\n                    <jats:italic>in vivo<\/jats:italic>\n                    administration of deuterium oxide. The\n                    <jats:sup>2<\/jats:sup>\n                    H enrichments of plasma glucose at different C\u2010H positions were measured as aldonitrile pentaacetate (AAc) and methyloxime\u2010trimethylsilyl (MoxTMS) derivatives or HMT by gas chromatography\/mass spectrometry (GC\/MS). Two pre\u2010ruminating fasted Holstein calves (51\u2009kg body mass, BM, age 7 days) received two oral bolus doses of\n                    <jats:sup>2<\/jats:sup>\n                    H\n                    <jats:sub>2<\/jats:sub>\n                    O (10\u2009g\/kg BM, 70 atom%\n                    <jats:sup>2<\/jats:sup>\n                    H) at 7:00\u2009h and 11:00\u2009h after overnight food withdrawal. Blood samples for fractional GNG determination were collected at \u221224 and between 6 and 9\u2009h after the first\n                    <jats:sup>2<\/jats:sup>\n                    H\n                    <jats:sub>2<\/jats:sub>\n                    O dose. The ratio of\n                    <jats:sup>2<\/jats:sup>\n                    H enrichments C5\/C2 represents the contribution of GNG to GP. The\n                    <jats:sup>2<\/jats:sup>\n                    H enrichment at C2 was calculated based on the ion fragments at\n                    <jats:italic>m\/z<\/jats:italic>\n                    328 (C1\u2010C6) \u2010\n                    <jats:italic>m\/z<\/jats:italic>\n                    187 (C3\u2010C6) of glucose AAc. The\n                    <jats:sup>2<\/jats:sup>\n                    H enrichment at C5 was approximated either by averaging the\n                    <jats:sup>2<\/jats:sup>\n                    H enrichment at C5\u2010C6 using the ion fragment of glucose MoxTMS at\n                    <jats:italic>m\/z<\/jats:italic>\n                    205 or by conversion of the C5 of glucose into HMT. The fractional GNG calculated by the C5\u2010C6 average\n                    <jats:sup>2<\/jats:sup>\n                    H enrichment method (41.4\u2009\u00b1\u20096.9%) compared to the HMT method (34.3\u2009\u00b1\u200911.4%) was not different (mean\u2009\u00b1\u2009SD, n\u2009=\u20096 replicates). In conclusion, GNG can be estimated with less laborious sample preparation by means of our new C5\u2010C6 average\n                    <jats:sup>2<\/jats:sup>\n                    H enrichment method using AAc and MoxTMS glucose derivatives. Copyright \u00a9 2010 John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/rcm.4509","type":"journal-article","created":{"date-parts":[[2010,4,6]],"date-time":"2010-04-06T20:32:59Z","timestamp":1270585979000},"page":"1287-1295","source":"Crossref","is-referenced-by-count":18,"title":["A simplified mass isotopomer approach to estimate gluconeogenesis rate\n                    <i>in vivo<\/i>\n                    using deuterium oxide"],"prefix":"10.1002","volume":"24","author":[{"given":"Peter","family":"Junghans","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Solvig","family":"G\u00f6rs","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iris S.","family":"Lang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia","family":"Steinhoff","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harald M.","family":"Hammon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cornelia C.","family":"Metges","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,4,7]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0029665199001275"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(92)90238-3"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117635"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9888(199805)33:5<444::AID-JMS651>3.0.CO;2-B"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.2001.280.1.E23"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.52.10.2475"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1996.270.4.E709"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.2000.278.1.E140"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.86.5.2220"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.24.14452"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.1195"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118803"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00601.2004"},{"key":"e_1_2_6_15_2","doi-asserted-by":"crossref","first-page":"E717","DOI":"10.1152\/ajpcell.1999.277.4.C717","volume":"277","author":"Chandramouli V","year":"1999","journal-title":"Am. 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