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This study describes the development of an analytical method for the quantitative determination of the concentration of hepcidin in clinical samples. The fragmentation of hepcidin was investigated using triple quadrupole and linear ion trap mass spectrometers. A standard quantity of a stable isotopically labelled hepcidin internal standard was added to serum samples. Extraction was performed by protein precipitation and weak cation\u2010exchange magnetic nanoparticles. Chromatography was carried out on sub 2\u2009\u00b5m particle stationary phase, using ultra\u2010high\u2010pressure liquid chromatography and a linear ion trap for quantitation. The lower limit of quantitation was 0.4\u2009nmol\/L with less than 20% accuracy and precision. The mean hepcidin concentration in sera for controls was 4.6\u2009\u00b1\u20092.7\u2009nmol\/L, in patients with sickle cell disease, 7.0\u2009\u00b1\u20098.9\u2009nmol\/L; in patients with end\u2010stage renal disease, 30.5\u2009\u00b1\u200915.7\u2009nmol\/L; and patients with penetrant hereditary hemochromatosis, 1.4\u2009\u00b1\u20090.8\u2009nmol\/L. Copyright \u00a9 2010 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/rcm.4512","type":"journal-article","created":{"date-parts":[[2010,4,6]],"date-time":"2010-04-06T20:30:36Z","timestamp":1270585836000},"page":"1251-1259","source":"Crossref","is-referenced-by-count":44,"title":["Quantitation of hepcidin in serum using ultra\u2010high\u2010pressure liquid chromatography and a linear ion trap mass spectrometer"],"prefix":"10.1002","volume":"24","author":[{"given":"Sukhvinder S.","family":"Bansal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincenzo","family":"Abbate","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian","family":"Bomford","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John 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