{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T04:04:48Z","timestamp":1791086688235,"version":"4.1.0"},"reference-count":16,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2010,12,14]],"date-time":"2010-12-14T00:00:00Z","timestamp":1292284800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2011,1,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The concept of protein cross\u2010linking in combination with mass spectrometry holds great promise to derive structural information on protein conformation and protein\u2010protein interactions. We recently presented a dissociative amine\u2010reactive cross\u2010linker (NHS\u2010BuUrBu\u2010NHS) that is shown herein to be universally applicable to protein structure analysis under matrix\u2010assisted laser desorption\/ionization tandem mass spectrometric (MALDI\u2010MS\/MS) conditions, based on the examples of the peptides substance P, luteinizing hormone releasing hormone (LHRH), and the 32\u2010kDa ligand\u2010binding domain of peroxisome proliferator\u2010activated receptor alpha (PPAR\u03b1). The characteristic fragment ion patterns and constant neutral losses of the cross\u2010linker greatly simplify the identification of different cross\u2010linked species from complex mixtures and drastically reduce the potential of identifying false\u2010positive cross\u2010links. Therefore, this cross\u2010linker holds an enormous potential for deriving structural information of proteins and protein complexes in a highly automated fashion. Copyright \u00a9 2010 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/rcm.4812","type":"journal-article","created":{"date-parts":[[2010,12,14]],"date-time":"2010-12-14T15:03:06Z","timestamp":1292338986000},"page":"155-161","source":"Crossref","is-referenced-by-count":26,"title":["A universal matrix\u2010assisted laser desorption\/ionization cleavable cross\u2010linker for protein structure analysis"],"prefix":"10.1002","volume":"25","author":[{"given":"Mathias Q.","family":"M\u00fcller","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes J.","family":"Zeiser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Dreiocker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Pich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathias","family":"Sch\u00e4fer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Sinz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,12,14]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-009-3405-5"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/mas.20082"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/jms.559"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(03)00721-6"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1039\/b801810c"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.R000001-MCP201"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1255\/ejms.748"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac101241t"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1044-0305(03)00327-1"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/jms.1702"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/jms.1775"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-003-2057-0"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac990809y"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2008.07.016"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-008-2231-5"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/jms.1544"}],"container-title":["Rapid Communications in Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Frcm.4812","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Frcm.4812","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rcm.4812","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T16:30:11Z","timestamp":1761496211000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/rcm.4812"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,12,14]]},"references-count":16,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2011,1,15]]}},"alternative-id":["10.1002\/rcm.4812"],"URL":"https:\/\/doi.org\/10.1002\/rcm.4812","archive":["Portico"],"relation":{},"ISSN":["0951-4198","1097-0231"],"issn-type":[{"value":"0951-4198","type":"print"},{"value":"1097-0231","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,12,14]]}}}