{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T21:08:45Z","timestamp":1761512925994,"version":"build-2065373602"},"reference-count":14,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2011,1,13]],"date-time":"2011-01-13T00:00:00Z","timestamp":1294876800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2011,2,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The development of reliable analytic methods, capable of separating mixtures of secondary metabolites as well as providing structural information, is essential for the investigation of secondary metabolites, e.g. from\n                    <jats:italic>Streptomyces<\/jats:italic>\n                    . Here we report a liquid chromatography\/electrospray ionization tandem mass spectrometry (LC\/ESI\u2010MS\/MS) method using a triple quadrupole mass analyzer for the structural elucidation of caprazamycins and liposidomycins from culture extracts of the wild\u2010type producer strains. Comparison of the fragmentation patterns in positive as well as in negative ionization mode revealed several characteristic product ions used for identification of six new caprazamycins. Furthermore, a chromatographic method for the purification of nucleosides from cell cultures using a boronic acid gel was adapted for the partial purification of the culture extracts. Copyright \u00a9 2011 John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/rcm.4889","type":"journal-article","created":{"date-parts":[[2011,1,24]],"date-time":"2011-01-24T01:25:19Z","timestamp":1295832319000},"page":"495-502","source":"Crossref","is-referenced-by-count":7,"title":["Identification and structural elucidation of new caprazamycins from\n                    <i>Streptomyces<\/i>\n                    sp. MK730\u201362F2 by liquid chromatography\/electrospray ionization tandem mass spectrometry"],"prefix":"10.1002","volume":"25","author":[{"given":"Stefanie","family":"Siebenberg","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonard","family":"Kaysser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emmanuel","family":"Wemakor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lutz","family":"Heide","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bertolt","family":"Gust","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernd","family":"Kammerer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,1,13]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.56.580"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/ja.2005.41"},{"key":"e_1_2_5_4_2","unstructured":"T.Takeuchi M.Igaraschi H.Naganawa M.Hamada.Antibiotic caprazamycins and process for producing the same.US Patent 6 780 616 B1 (2004)."},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.7.1640"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1039\/b202149h"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00865a012"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.38.1617"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200900637"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M901258200"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(96)00757-1"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2091-8-25"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1021\/jo00050a009"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.51.647"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.52.281"}],"container-title":["Rapid Communications in Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Frcm.4889","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Frcm.4889","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rcm.4889","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T09:47:30Z","timestamp":1761472050000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/rcm.4889"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,1,13]]},"references-count":14,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2011,2,28]]}},"alternative-id":["10.1002\/rcm.4889"],"URL":"https:\/\/doi.org\/10.1002\/rcm.4889","archive":["Portico"],"relation":{},"ISSN":["0951-4198","1097-0231"],"issn-type":[{"type":"print","value":"0951-4198"},{"type":"electronic","value":"1097-0231"}],"subject":[],"published":{"date-parts":[[2011,1,13]]}}}