{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T15:34:46Z","timestamp":1765294486518,"version":"build-2065373602"},"reference-count":20,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2011,4,12]],"date-time":"2011-04-12T00:00:00Z","timestamp":1302566400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2011,5,15]]},"abstract":"<jats:p>\n                    Vitamin D plays important roles in bone health and a variety of other pathophysiological conditions. 1\u03b1,25\u2010Dihydroxyvitamin D is the active form of vitamin D. Quantification of serum 1\u03b1,25\u2010dihydroxyvitamin D is useful for evaluation of several diseases including chronic renal failure, hypoparathyroidism, sarcoidosis, and rickets. Measurement of 1\u03b1,25\u2010dihydroxyvitamin D is very challenging due to its low circulating concentration and presence of interfering substances in serum. In this report, a liquid chromatography\/tandem mass spectrometry (LC\/MS\/MS) method for quantifying serum 1\u03b1,25\u2010dihydroxyvitamin D is described. Lithium adducts of 1\u03b1,25\u2010dihydroxyvitamin D were formed prior to mass spectrometry analysis to improve ionization efficiency. We tested a number of different sample preparation procedures and found that immunoaffinity extraction was the method of choice because it completely removed isobaric interferences and matrix effects present in patient serum. Extraction efficiency, expressed as absolute recovery, was greater than 60% in both patient serum and charcoal\u2010stripped serum. This method was linear from 3.4 to 206.2\u2009pg\/mL for 1\u03b1,25\u2010dihydroxyvitamin D\n                    <jats:sub>3<\/jats:sub>\n                    and 3.9 to 212.6\u2009pg\/mL for 1\u03b1,25\u2010dihydroxyvitamin D\n                    <jats:sub>2<\/jats:sub>\n                    with an accuracy of 89.8\u201398.4% and 97.5\u2013115.7%, respectively. Inter\u2010assay and intra\u2010assay coefficients of variance (CVs) for both analytes at two different concentration levels ranged from 2.5\u20137.0%. Comparison with a radioimmunoassay for measuring total 1\u03b1,25\u2010dihydroxyvitamin D concentration using 40 patient samples showed a Deming regression slope of 0.751, a y\u2010intercept of 0.84\u2009pg\/mL, an r value of 0.7909, and a mean percentage difference of \u201327.1%. Comparison with a reference LC\/MS\/MS method (n\u2009=\u200920) showed a Deming regression slope of 1.020, a y\u2010intercept of 1.32\u2009pg\/mL, an r value of 0.9797, and a mean percentage difference of \u20132.9%. In conclusion, usage of immunoaffinity extraction enabled a sensitive LC\/MS\/MS method for quantification of 1\u03b1,25\u2010dihydroxyvitamin D in serum. Copyright \u00a9 2011 John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/rcm.4988","type":"journal-article","created":{"date-parts":[[2011,4,12]],"date-time":"2011-04-12T09:24:20Z","timestamp":1302600260000},"page":"1241-1249","source":"Crossref","is-referenced-by-count":54,"title":["Sensitive measurement of serum 1\u03b1,25\u2010dihydroxyvitamin D by liquid chromatography\/tandem mass spectrometry after removing interference with immunoaffinity extraction"],"prefix":"10.1002","volume":"25","author":[{"given":"Chao","family":"Yuan","sequence":"first","affiliation":[{"name":"Department of Clinical Pathology Cleveland Clinic  Cleveland OH 44195 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Justin","family":"Kosewick","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology Cleveland Clinic  Cleveland OH 44195 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiang","family":"He","sequence":"additional","affiliation":[{"name":"ThermoFisher Scientific  355 River Oaks Parkway San Jose CA 95134 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta","family":"Kozak","sequence":"additional","affiliation":[{"name":"ThermoFisher Scientific  355 River Oaks Parkway San Jose CA 95134 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sihe","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology Cleveland Clinic  Cleveland OH 44195 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,4,12]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra070553"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0954422409990151"},{"volume-title":"Tietz Textbook of Clinical Chemistry and Molecular Diagnostics","year":"2006","author":"Endres D. 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