{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T14:06:21Z","timestamp":1761487581693,"version":"build-2065373602"},"reference-count":19,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2001,10,29]],"date-time":"2001-10-29T00:00:00Z","timestamp":1004313600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2001,12,15]]},"abstract":"Abstract<\/jats:title>\n \n The disulfide bond pattern of\n Trimeresurus stejnegeri<\/jats:italic>\n lectin (TSL), a new member of the C\u2010type lectin family, was determined by mass spectrometry. Four intrachain disulfide bonds of TSL, Cys\n 3<\/jats:sup>\n \u2010Cys\n 14<\/jats:sup>\n , Cys\n 31<\/jats:sup>\n \u2010Cys\n 131<\/jats:sup>\n , Cys\n 38<\/jats:sup>\n \u2010Cys\n 133<\/jats:sup>\n and Cys\n 106<\/jats:sup>\n \u2010Cys\n 123<\/jats:sup>\n , and two interchain linkages, Cys\n 2<\/jats:sup>\n \u2010Cys\n 2<\/jats:sup>\n and Cys\n 86<\/jats:sup>\n \u2010Cys\n 86<\/jats:sup>\n , were determined. Three strategies were used in this work. One intrachain (Cys\n 106<\/jats:sup>\n \u2010Cys\n 123<\/jats:sup>\n ) and one interchain (Cys\n 86<\/jats:sup>\n \u2010Cys\n 86<\/jats:sup>\n ) disulfide linkages were detected by standard MS methods. The disulfide bonds Cys\n 2<\/jats:sup>\n \u2010Cys\n 2<\/jats:sup>\n and Cys\n 3<\/jats:sup>\n \u2010Cys\n 14<\/jats:sup>\n were analyzed using a modified partial reduction procedure and MS\/MS. The last two disulfide bonds were characterized by a MS\/MS\/MS technique. The strategies developed in this work could be applied more generally to detection of disulfide bond patterns. Copyright \u00a9 2001 John Wiley & Sons, Ltd.\n <\/jats:p>","DOI":"10.1002\/rcm.500","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T12:22:28Z","timestamp":1030278148000},"page":"2213-2220","source":"Crossref","is-referenced-by-count":7,"title":["Determination of the disulfide bond pattern of a novel C\u2010type lectin from snake venom by mass spectrometry"],"prefix":"10.1002","volume":"15","author":[{"given":"Rong","family":"Zeng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiang","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao\u2010Xia","family":"Shao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ke\u2010Yi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi\u2010Chang","family":"Xia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,10,29]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(84)07021-X"},{"key":"e_1_2_6_3_2","series-title":"Hydrolytic Enzymes","first-page":"39","author":"Brocklehurst K","year":"1987"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00429a058"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560021018"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1996.0108"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560060215"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi981082v"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.11.7207"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac9707693"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1997.t01-1-00543.x"},{"key":"e_1_2_6_12_2","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1016\/0959-440X(95)80052-2","volume":"5","author":"Drickamer K","journal-title":"Curr. 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