{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T05:47:50Z","timestamp":1777441670277,"version":"3.51.4"},"reference-count":29,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2011,10,13]],"date-time":"2011-10-13T00:00:00Z","timestamp":1318464000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2011,11,30]]},"abstract":"<jats:p>\n                    We describe a new, simple, robust and efficient method based on direct\u2010tissue matrix\u2010assisted laser desorption ionization time\u2010of\u2010flight (MALDI\u2010TOF) mass spectrometry that enables consistent semi\u2010quantitation of peptide hormones in isolated pancreatic islets from normal and diabetic rodents. Prominent signals were measured that corresponded to all the main peptide hormones present in islet\u2010endocrine cells: (\u03b1\u2010cells) glucagon, glicentin\u2010related polypeptide\/GRPP; (\u03b2\u2010cells) insulin I, insulin II, C\u2010peptide I, C\u2010peptide II, amylin; (\u03b4\u2010cells) somatostatin\u201014; and (PP\u2010cells), and pancreatic polypeptide. The signal ratios coincided with known relative hormone abundances. The method demonstrated that severe insulin deficiency is accompanied by elevated levels of all non\u2010\u03b2\u2010cell\u2010hormones in diabetic rat islets, consistent with alleviation of paracrine suppression of hormone production by non\u2010\u03b2\u2010cells. It was also effective in characterizing hormonal phenotype in hemizygous human\u2010amylin transgenic mice that express human and mouse amylin in approx. equimolar quantities. Finally, the method demonstrated utility in basic peptide\u2010hormone discovery by identifying a prominent new\n                    <jats:italic>Gcg<\/jats:italic>\n                    \u2010gene\u2010derived peptide (theoretical monoisotopic molecular weight 3263.5\u2009Da), closely related to but distinct from GRPP, in diabetic islets. This peptide, whose sequence is HAPQDTEENARSFPASQTEPLEDPNQINE in\n                    <jats:italic>Rattus norvegicus<\/jats:italic>\n                    , could be a peptide hormone whose roles in physiology and metabolic disease warrant further investigation. This method provides a powerful new approach that could provide important new insights into the physiology and regulation of peptide hormones in islets and other endocrine tissues. It has potentially wide\u2010ranging applications that encompass endocrinology, pharmacology, phenotypic analysis in genetic models of metabolic disease, and hormone discovery, and could also effectively limit the numbers of animals required for such studies. Copyright \u00a9 2011 John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/rcm.5239","type":"journal-article","created":{"date-parts":[[2011,10,20]],"date-time":"2011-10-20T06:07:27Z","timestamp":1319090847000},"page":"3387-3395","source":"Crossref","is-referenced-by-count":11,"title":["A simple and rapid method for identifying and semi\u2010quantifying peptide hormones in isolated pancreatic islets by direct\u2010tissue matrix\u2010assisted laser desorption ionization time\u2010of\u2010flight mass spectrometry"],"prefix":"10.1002","volume":"25","author":[{"given":"Kevin W.","family":"Stewart","sequence":"first","affiliation":[{"name":"The Waikato Institute of Technology  Hamilton New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony R. J.","family":"Phillips","sequence":"additional","affiliation":[{"name":"Department of Surgery, Faculty of Medical and Health Sciences University of Auckland  Auckland New Zealand"},{"name":"School of Biological Sciences, Faculty of Science University of Auckland  Auckland New Zealand"},{"name":"Maurice Wilkins Centre for Molecular Biodiscovery University of Auckland  Auckland New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lynda","family":"Whiting","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, Faculty of Science University of Auckland  Auckland New Zealand"},{"name":"Maurice Wilkins Centre for Molecular Biodiscovery University of Auckland  Auckland New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mia","family":"J\u00fcllig","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, Faculty of Science University of Auckland  Auckland New Zealand"},{"name":"Maurice Wilkins Centre for Molecular Biodiscovery University of Auckland  Auckland New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin J.","family":"Middleditch","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, Faculty of Science University of Auckland  Auckland New Zealand"},{"name":"Maurice Wilkins Centre for Molecular Biodiscovery University of Auckland  Auckland New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Garth J. S.","family":"Cooper","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, Faculty of Science University of Auckland  Auckland New Zealand"},{"name":"Maurice Wilkins Centre for Molecular Biodiscovery University of Auckland  Auckland New Zealand"},{"name":"Centre for Advanced Discovery and Experimental Therapeutics, NIHR Manchester Biomedical Research Centre Central Manchester University Hospitals NHS Foundation Trust, and The School of Biomedicine, The Medical School, University of Manchester  Manchester M13 9WL UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,10,13]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4419-6956-9_14"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr800675k"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.3253"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1998.tb10789.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac060788p"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac970888i"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200800237"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac048323r"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.2337\/db06-1755"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.2337\/db09-0548"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-006-0600-5"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/bfgp\/eln041"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac990781q"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.2259"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.24.11290"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.anchem.1.031207.112841"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.38.23222"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac00086a023"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1290091216"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2006.06.002"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.12.6591"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197808312990901"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.139.11.6294"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.30.4.358"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-109-4-1107"},{"key":"e_1_2_6_27_1","first-page":"275","article-title":"Pancreatic polypeptide (author's transl)","volume":"4","author":"Gepts W.","year":"1978","journal-title":"Diabete Metab."},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02065429"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1999.276.1.E19"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.54.6.1808"}],"container-title":["Rapid Communications in Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Frcm.5239","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Frcm.5239","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rcm.5239","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T15:44:26Z","timestamp":1761493466000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/rcm.5239"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,10,13]]},"references-count":29,"journal-issue":{"issue":"22","published-print":{"date-parts":[[2011,11,30]]}},"alternative-id":["10.1002\/rcm.5239"],"URL":"https:\/\/doi.org\/10.1002\/rcm.5239","archive":["Portico"],"relation":{},"ISSN":["0951-4198","1097-0231"],"issn-type":[{"value":"0951-4198","type":"print"},{"value":"1097-0231","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,10,13]]}}}