{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T12:40:20Z","timestamp":1791117620730,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2002,1,25]],"date-time":"2002-01-25T00:00:00Z","timestamp":1011916800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2002,3,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Animal venoms are important sources of novel pharmacological tools, useful in biochemical characterization of their receptors. Venom quality control, batch\u2010to\u2010batch homogeneity and high reproducibility of venom fractionation and toxin purification are crucial issues for biochemical and pharmacological studies. To address these issues, a study of the variability of tarantula spider venom samples was undertaken. Venom profiles of samples collected from individuals of different age and sex, and from sibling spiders of the same species, were generated by high\u2010performance liquid chromatography (HPLC) and matrix\u2010assisted laser desorption\/ionization time\u2010of\u2010flight mass spectrometry (MALDI\u2010TOFMS) and analyzed to assess venom variability and method accuracy. Sex\u2010linked venom variation was studied on eight species. Clear qualitative differences were observed for six out of eight species, as well as quantitative differences. Age\u2010related variation studied in\n                    <jats:italic>Poecilotheria rufilata<\/jats:italic>\n                    showed essentially age\u2010related quantitative differences between adults of both sexes and immature juveniles. The venoms of nine siblings and three wild\u2010collected\n                    <jats:italic>Pterinochilus murinus<\/jats:italic>\n                    were studied for individual variation, showing only very minor quantitative differences. On the same samples, the quality of MALDI\u2010TOFMS venom fingerprinting was demonstrated to be highly reproducible. Our results show that tarantula venom peptide fingerprinting is a highly reliable identification method, that pooled batches of venom from several animals can be used for venom purification, that venom composition does not appear to be qualitatively related to ontogenesis in the spiders studied, and that qualitative sex\u2010linked variation occurs across most species and may be important in activity studies. Copyright \u00a9 2002 John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/rcm.595","type":"journal-article","created":{"date-parts":[[2002,9,10]],"date-time":"2002-09-10T21:59:00Z","timestamp":1031695140000},"page":"403-413","source":"Crossref","is-referenced-by-count":55,"title":["Matrix\u2010assisted laser desorption\/ionization time\u2010of\u2010flight mass spectrometry and high\u2010performance liquid chromatography study of quantitative and qualitative variation in tarantula spider venoms"],"prefix":"10.1002","volume":"16","author":[{"given":"Pierre","family":"Escoubas","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerardo","family":"Corzo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian J.","family":"Whiteley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie\u2010Louise","family":"C\u00e9l\u00e9rier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terumi","family":"Nakajima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,1,25]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-9084(00)01166-4"},{"key":"e_1_2_6_3_2","first-page":"277","volume":"90","author":"Nkinin SW","year":"1997","journal-title":"Bull. 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