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These metabolites have been characterised quite well in rodents and fish, but available information in humans remains scarce. For their identification, an efficient method for the simultaneous analysis of PBDEs, hydroxylated PBDEs (OH\u2010PBDEs), and other PBDE metabolites in a single run was needed and has been developed in this work. Atmospheric pressure ionisation modes were compared, and Atmospheric Pressure Photo\u2010Ionization (APPI) was selected. After careful setting of APPI parameters such as dopant and operating temperature, the optimised method was based on APPI ionization coupled to High\u2010Resolution Mass Spectrometry operating in the full scan mode at a resolution of 60 000. This provided excellent sensitivity and specificity, allowing the discrimination of signals which could not be resolved on a triple quadrupole used as a reference. The full\u2010scan high\u2010resolution acquisition mode allowed monitoring of both parent PBDEs and their metabolites, including hydroxylated PBDEs, with detection limits ranging from 0.1\u2009pg to 4.5\u2009pg injected on\u2010column based on the investigated standard compounds. The method was applied to the study of BDE\u201047 metabolism after incubation with human primary cultures of hepatocytes, and proved to be efficient not only for monitoring the parent compound and expected hydroxylated metabolites, but also for the identification of other non\u2010targeted metabolites. In addition to hydroxy\u2010BDE\u201047, several conjugated metabolites could be located, and the formation of a dihydrodiol derivative was evidenced for the first time in the case of PBDEs in this work. Copyright \u00a9 2012 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/rcm.6136","type":"journal-article","created":{"date-parts":[[2012,2,10]],"date-time":"2012-02-10T21:58:53Z","timestamp":1328911133000},"page":"599-610","source":"Crossref","is-referenced-by-count":18,"title":["Development of a liquid chromatography\/atmospheric pressure photo\u2010ionization high\u2010resolution mass spectrometry analytical method for the simultaneous determination of polybrominated diphenyl ethers and their metabolites: application to BDE\u201047 metabolism in human hepatocytes"],"prefix":"10.1002","volume":"26","author":[{"given":"Charlotte","family":"Marteau","sequence":"first","affiliation":[{"name":"INRA UMR 1331 Toxalim INRA\u2010INP  180 chemin de Tournefeuille, BP 93173 F31027 Toulouse cedex 3 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvie","family":"Chevolleau","sequence":"additional","affiliation":[{"name":"INRA UMR 1331 Toxalim INRA\u2010INP  180 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