{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T00:49:04Z","timestamp":1778546944532,"version":"3.51.4"},"reference-count":31,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2012,2,7]],"date-time":"2012-02-07T00:00:00Z","timestamp":1328572800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2012,3,30]]},"abstract":"\n RATIONALE<\/jats:title>\n \n F2\u2010isoprostanes are a series of prostaglandin F2\u2010like compounds that are formed by free\u2010radical\u2010catalyzed peroxidation of arachidonic acid (ARA). Several F2\u2010isoprostanes, but in particular 8\u2010epi\u2010PGF\n 2\u03b1<\/jats:sub>\n , are widely used as oxidative stress biomarkers. In this study we have developed an analytical tool for finger\u2010tip blood sampling and analysis of 8\u2010epi\u2010PGF\n 2\u03b1<\/jats:sub>\n from dried blood spots (DBS).\n <\/jats:p>\n <\/jats:sec>\n \n METHODS<\/jats:title>\n \n We have applied solid\u2010phase extraction (SPE) and liquid chromatography\/tandem mass spectrometry (LC\/MS\/MS) for the extraction, separation and detection of 8\u2010epi\u2010PGF\n 2\u03b1<\/jats:sub>\n in DBS and have studied the stability of this marker using the DBS collection platform.\n <\/jats:p>\n <\/jats:sec>\n \n RESULTS<\/jats:title>\n \n The mass limit of detection (mLOD) for 8\u2010epi\u2010PGF\n 2\u03b1<\/jats:sub>\n extracted from DBS samples was 1.5\u2009pg while the concentration limit of detection (cLOD) and concentration limit of quantitation (cLOQ) were 6\u2009pg\/mL and 18\u2009pg\/mL, respectively. All values based on triplicate analysis. Sufficient stability of 8\u2010epi\u2010PGF\n 2\u03b1<\/jats:sub>\n in DBS was achieved by preconditioning DBS paper with vitamin E and BHT.\n <\/jats:p>\n <\/jats:sec>\n \n CONCLUSIONS<\/jats:title>\n \n The developed method is sensitive, specific, robust, efficient, and can accurately measure endogenous concentrations of 8\u2010epi\u2010PGF\n 2\u03b1<\/jats:sub>\n in DBS. Thus, it offers an analytical approach to measure 8\u2010epi\u2010PGF\n 2\u03b1<\/jats:sub>\n by a novel sample collection technique that is less invasive and costly than conventional techniques. Copyright \u00a9 2012 John Wiley & Sons, Ltd.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/rcm.6149","type":"journal-article","created":{"date-parts":[[2012,2,10]],"date-time":"2012-02-10T21:58:53Z","timestamp":1328911133000},"page":"645-652","source":"Crossref","is-referenced-by-count":20,"title":["Dried blood spot (DBS) sample collection for determination of the oxidative stress biomarker 8\u2010epi\u2010PGF\n 2\u03b1<\/sub>\n in humans using liquid chromatography\/tandem mass spectrometry"],"prefix":"10.1002","volume":"26","author":[{"given":"Nasser E.","family":"Bastani","sequence":"first","affiliation":[{"name":"Institute of Basic Medical Sciences University of Oslo P.O. Box\u20091046 Blindern N\u20100316 Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas E.","family":"Gundersen","sequence":"additional","affiliation":[{"name":"Institute of Basic Medical Sciences University of Oslo P.O. Box\u20091046 Blindern N\u20100316 Oslo Norway"},{"name":"Vitas AS Oslo Innovation Park N\u20100349 Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rune","family":"Blomhoff","sequence":"additional","affiliation":[{"name":"Institute of Basic Medical Sciences University of Oslo P.O. Box\u20091046 Blindern N\u20100316 Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,2,7]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/00041433-200502000-00009"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2891-9-3"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-9-458"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-07-4535"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd1130"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M700097-JLR200"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2007.11.047"},{"key":"e_1_2_6_9_1","first-page":"171","volume-title":"Drugs of Natural Origin","author":"Samuelsson G.","year":"1992"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11745-008-3222-1"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2006.078972"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2011.02.020"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemphyslip.2003.09.011"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.03.024"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.04-2330rev"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.D600040-JLR200"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/13547500500216546"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(99)00264-6"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02253564"},{"key":"e_1_2_6_20_1","first-page":"625","volume":"16","author":"Morrow J. 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