{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T17:55:43Z","timestamp":1791136543059,"version":"4.1.0"},"reference-count":20,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2012,2,10]],"date-time":"2012-02-10T00:00:00Z","timestamp":1328832000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2012,3,30]]},"abstract":"<jats:sec>\n                    <jats:title>RATIONALE<\/jats:title>\n                    <jats:p>Chemical cross\u2010linking in combination with a mass spectrometric analysis of the created cross\u2010linked products is an area of growing interest for deriving low\u2010resolution structural information of proteins and protein complexes. One of the greatest challenges is the complexity of the created cross\u2010linking mixtures, which can be met by a charge\u2010based enrichment of cross\u2010linked peptides after proteolytic digestion using strong cation\u2010exchange (SCX) chromatography.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS<\/jats:title>\n                    <jats:p>\n                      SCX chromatography was used for the enrichment of cross\u2010linked peptides with the\n                      <jats:italic>N<\/jats:italic>\n                      \u2010hydroxysuccinimide ester bis(sulfosuccinimidyl)succinate (BS\n                      <jats:sup>3<\/jats:sup>\n                      ) prior to a mass spectrometric analysis by nano\u2010HPLC\/nano\u2010ESI\u2010LTQ\u2010Orbitrap\u2010MS\/MS. Bovine serum albumin (BSA) and glutathione S\u2010transferase (GST) were employed as model proteins.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Conditions for SCX enrichment were optimized for obtaining as many interpeptide cross\u2010linked peptides as possible in order to maximize the amount of structural information from a single experiment. With an SCX\u2010based enrichment step of cross\u2010linked products within BSA using the cross\u2010linker BS\n                      <jats:sup>3<\/jats:sup>\n                      , 154 interpeptidal cross\u2010linking products were identified during nano\u2010HPLC\/nano\u2010ESI\u2010MS\/MS analyses, whereas analyses without a prior SCX enrichment allowed the identification of merely 20 cross\u2010linked products. The application of the SCX enrichment strategy for the analysis of cross\u2010linked products of GST with BS\n                      <jats:sup>3<\/jats:sup>\n                      allowed the identification of 26 interpeptidal cross\u2010linked products compared with 16 without SCX enrichment.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS<\/jats:title>\n                    <jats:p>For both proteins investigated herein, BSA and GST, the introduction of an SCX\u2010based enrichment step prior to nano\u2010HPLC\/nano\u2010ESI\u2010MS\/MS of cross\u2010linked products led to a considerable gain in structural information. Copyright \u00a9 2012 John Wiley &amp; Sons, Ltd.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.6150","type":"journal-article","created":{"date-parts":[[2012,2,10]],"date-time":"2012-02-10T22:03:10Z","timestamp":1328911390000},"page":"653-658","source":"Crossref","is-referenced-by-count":91,"title":["Optimizing the enrichment of cross\u2010linked products for mass spectrometric protein analysis"],"prefix":"10.1002","volume":"26","author":[{"given":"Romy","family":"Fritzsche","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Chemistry &amp; Bioanalytics, Institute of Pharmacy Martin\u2010Luther University Halle\u2010Wittenberg  Wolfgang\u2010Langenbeck\u2010Str. 4 D\u201006120 Halle (Saale) Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian H.","family":"Ihling","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry &amp; Bioanalytics, Institute of Pharmacy Martin\u2010Luther University Halle\u2010Wittenberg  Wolfgang\u2010Langenbeck\u2010Str. 4 D\u201006120 Halle (Saale) Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"G\u00f6tze","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Biotechnology Martin\u2010Luther University Halle\u2010Wittenberg  Wolfgang\u2010Langenbeck\u2010Str. 4 D\u201006120 Halle (Saale) Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Sinz","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry &amp; Bioanalytics, Institute of Pharmacy Martin\u2010Luther University Halle\u2010Wittenberg  Wolfgang\u2010Langenbeck\u2010Str. 4 D\u201006120 Halle (Saale) Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,2,10]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.090099097"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(03)00721-6"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jms.1762"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/mas.20082"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-009-3405-5"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1039\/b801810c"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M800232-MCP200"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2010.10.014"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/mas.20293"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/381290a0"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jasms.2004.01.011"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M700274-MCP200"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1192"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.R000001-MCP201"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1044-0305(03)00327-1"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13361-011-0261-2"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2009.401"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr200260n"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1586\/epr.10.46"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-9639(02)00414-4"}],"container-title":["Rapid Communications in Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Frcm.6150","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rcm.6150","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T09:34:07Z","timestamp":1761471247000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/rcm.6150"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,2,10]]},"references-count":20,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2012,3,30]]}},"alternative-id":["10.1002\/rcm.6150"],"URL":"https:\/\/doi.org\/10.1002\/rcm.6150","archive":["Portico"],"relation":{},"ISSN":["0951-4198","1097-0231"],"issn-type":[{"value":"0951-4198","type":"print"},{"value":"1097-0231","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,2,10]]}}}