{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T11:07:10Z","timestamp":1761476830846,"version":"build-2065373602"},"reference-count":36,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2012,2,23]],"date-time":"2012-02-23T00:00:00Z","timestamp":1329955200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2012,4,15]]},"abstract":"<jats:sec>\n                    <jats:title>RATIONALE<\/jats:title>\n                    <jats:p>Iberian ham is a product of high commercial value whose quality mainly depends on breeding and feeding of pigs in an authorized way. Simple, fast, simple, reliable and high\u2010throughput analytical methods are necessary to assure the quality of ham and for fraud prevention. Tandem mass spectrometry (MS\/MS) is proposed as an advantageous alternative over other analytical techniques commonly used in this industry for product authentication.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS<\/jats:title>\n                    <jats:p>The analytical approach is based on direct infusion electrospray mass spectrometry (ESI(+)\u2010MS) of dichloromethane\/methanol (60:40) extracts of ham intramuscular fat. Similarly, atmospheric pressure photoionization ionization mass spectrometry (APPI(+)\u2010MS) was used with a flow injection analysis system for sample introduction with methanol\/water (50%) as the mobile phase and toluene as the dopant. All experiments were performed on an API QSTAR\u00ae XL Hybrid system using both ESI and APPI sources.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      The ESI(+)\u2010MS mass spectra present several clusters of peaks attributed to ammonium adducts [M\u2009+\u2009NH\n                      <jats:sub>4<\/jats:sub>\n                      <jats:sup>+<\/jats:sup>\n                      ] of lipid compounds (mono\u2010, di\u2010 and triacylglycerols\n                      <jats:styled-content>\u2013<\/jats:styled-content>\n                      MGs, DGs, TGs, and free fatty acids \u2013 FFAs), that can be identified by MS\/MS spectra. On the other hand, the APPI(+)\u2010MS spectra present [M\u2009+\u2009H\n                      <jats:sup>+<\/jats:sup>\n                      ] ions and reflect a higher fragmentation of the sample. Five different types of Iberian ham samples were successfully classified using partial least\u2010squares discriminant analysis (PLS\u2010DA) of data from these samples.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS<\/jats:title>\n                    <jats:p>The application of direct infusion tandem mass spectrometry to dichloromethane\/methanol extracts from intramuscular fat ham allows the simple, fast and reliable fingerprinting typification of different Iberian ham samples from pigs with different diets. With the proposed method, sample handling is minimal and chromatographic separation is not necessary, which represents an evident advantage over other analytical procedures usually used for this purpose. Copyright \u00a9 2012 John Wiley &amp; Sons, Ltd.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.6155","type":"journal-article","created":{"date-parts":[[2012,2,24]],"date-time":"2012-02-24T22:47:15Z","timestamp":1330123635000},"page":"835-844","source":"Crossref","is-referenced-by-count":20,"title":["Iberian ham typification by direct infusion electrospray and photospray ionization mass spectrometry fingerprinting"],"prefix":"10.1002","volume":"26","author":[{"given":"R.","family":"Gonz\u00e1lez\u2010Dominguez","sequence":"first","affiliation":[{"name":"Department of Chemistry and Material Science, Faculty of Experimental Science University of Huelva  21007 Huelva Spain"},{"name":"Campus of Excellence CEIA3 University of Huelva  21007 Huelva Spain"},{"name":"Research Center on Health and Environment (CYSMA) University of Huelva  21007 Huelva Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Garc\u00eda\u2010Barrera","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Material Science, Faculty of Experimental Science University of Huelva  21007 Huelva Spain"},{"name":"Campus of Excellence CEIA3 University of Huelva  21007 Huelva Spain"},{"name":"Research Center on Health and Environment (CYSMA) University of Huelva  21007 Huelva Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.\u2010L.","family":"G\u00f3mez\u2010Ariza","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Material Science, Faculty of Experimental Science University of Huelva  21007 Huelva Spain"},{"name":"Campus of Excellence CEIA3 University of Huelva  21007 Huelva Spain"},{"name":"Research Center on Health and Environment (CYSMA) University of Huelva  21007 Huelva Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,2,23]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0309-1740(01)00143-7"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1745-4557.1996.tb00434.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0309-1740(96)00102-7"},{"key":"e_1_2_6_5_1","first-page":"178","article-title":"Evolution of different physico\u2010chemical parameters during ripening Iberian ham from Iberian (100%) and Iberian Duroc pigs (50%)","volume":"34","author":"Antequera T.","year":"1994","journal-title":"Rev. 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