{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T03:00:06Z","timestamp":1780628406641,"version":"3.54.1"},"reference-count":59,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2012,5,4]],"date-time":"2012-05-04T00:00:00Z","timestamp":1336089600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2012,6,30]]},"abstract":"<jats:sec>\n                    <jats:title>RATIONALE<\/jats:title>\n                    <jats:p>Busulfan (Bu) is an important component of the myeloablative conditioning regimen prior to hematopoietic stem cell transplantation (HSCT) especially in children. Intravenously administered Bu exhibits a therapeutic window phenomenon requiring therapeutic drug monitoring. Analytical methods developed for Bu routine monitoring were aimed at using low volumes of biological fluids and development of simple procedures to facilitate the dosage adjustment. In this report, we describe a simple, rapid method for Bu measurement using dried blood spots (DBS) from only 5\u2009\u03bcL of whole blood.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS<\/jats:title>\n                    <jats:p>Bu extracted from DBS with methanol was measured by high\u2010performance liquid chromatography with electrospray ionization and tandem mass spectrometry in multiple reaction monitoring mode using D8\u2010Bu as an internal standard. The method was in\u2010house validated evaluating trueness, repeatability, within\u2010laboratory reproducibility, specificity and the lower limit of quantification (LLOQ).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      The method was linear in the calibration range of 100\u20132000\u2009ng\u2009mL\n                      <jats:sup>\u20131<\/jats:sup>\n                      (r\n                      <jats:sup>2<\/jats:sup>\n                      &gt;0.99) encompassing the therapeutic concentrations of Bu. A good trueness (&lt;14%), precision (&lt;10%), and recovery (100%) were observed during validation of the method with quality controls of 300, 600 and 1400\u2009ng\u2009mL\n                      <jats:sup>\u20131<\/jats:sup>\n                      . The LLOQ was determined as 50\u2009ng\u2009mL\n                      <jats:sup>\u20131<\/jats:sup>\n                      and no matrix or carryover effects were observed. The validated method was applied to measure Bu levels in four children receiving infusion of Bu prior to HSCT. A good correlation was observed between the Bu levels measured by DBS and dried plasma spot (DPS) (r\n                      <jats:sup>2<\/jats:sup>\n                      \u2009=\u20090.96) and between DPS and the GC\/MS method (r\n                      <jats:sup>2<\/jats:sup>\n                      \u2009=\u20090.92). Bu was found to be stable in DBS up to 6\u2009h at room temperature and for 24\u2009h at 4\u00b0C.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS<\/jats:title>\n                    <jats:p>The new DBS method facilitates earlier dosage adjustment during Bu therapy by its specific and simple procedure using 5\u2009\u03bcL of whole blood. Copyright \u00a9 2012 John Wiley &amp; Sons, Ltd.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.6241","type":"journal-article","created":{"date-parts":[[2012,5,7]],"date-time":"2012-05-07T00:57:22Z","timestamp":1336352242000},"page":"1437-1446","source":"Crossref","is-referenced-by-count":40,"title":["A simplified method for busulfan monitoring using dried blood spot in combination with liquid chromatography\/tandem mass spectrometry"],"prefix":"10.1002","volume":"26","author":[{"given":"Marc","family":"Ansari","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Onco\u2010hematology Unit Geneva University Hospitals  Geneva Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chakradhara Rao S.","family":"Uppugunduri","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Onco\u2010hematology Unit Geneva University Hospitals  Geneva Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julien","family":"D\u00e9glon","sequence":"additional","affiliation":[{"name":"Unit of Toxicology University Center of Legal Medicine  Geneva Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yves","family":"Th\u00e9or\u00eat","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, CHU Sainte Justine University of Montreal  Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Versace","sequence":"additional","affiliation":[{"name":"Unit of Toxicology University Center of Legal Medicine  Geneva Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabienne","family":"Gumy\u2010Pause","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Onco\u2010hematology Unit Geneva University Hospitals  Geneva Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hulya","family":"Ozsahin","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Onco\u2010hematology Unit Geneva University Hospitals  Geneva Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Dayer","sequence":"additional","affiliation":[{"name":"Clinical Pharmacology and Toxicology Service Geneva University Hospitals  Geneva Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jules","family":"Desmeules","sequence":"additional","affiliation":[{"name":"Clinical Pharmacology and Toxicology Service Geneva University Hospitals  Geneva Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youssef","family":"Daali","sequence":"additional","affiliation":[{"name":"Clinical Pharmacology and Toxicology Service Geneva University Hospitals  Geneva Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,5,4]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198312013092202"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V84.6.2036.2036"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V85.8.2263.bloodjournal8582263"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V91.3.756"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.cco.0000245318.90015.72"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/bmt.2008.54"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.21630"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V84.7.2144.2144"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-11-3778"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1704209"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1704446"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2006.10.026"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/bmt.2009.143"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3046.2011.01529.x"},{"key":"e_1_2_6_16_1","first-page":"31","article-title":"Graft\u2010rejection and toxicity following bone marrow transplantation in relation to busulfan pharmacokinetics","volume":"16","author":"Slattery J. 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