{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T17:11:54Z","timestamp":1791047514032,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2012,7,19]],"date-time":"2012-07-19T00:00:00Z","timestamp":1342656000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2012,9,15]]},"abstract":"<jats:sec>\n                    <jats:title>RATIONALE<\/jats:title>\n                    <jats:p>\n                      In developing countries,\n                      <jats:italic>Shigella flexneri<\/jats:italic>\n                      (\n                      <jats:italic>Sf)<\/jats:italic>\n                      is the major causative agent of the endemic shigellosis (bacillary dysentery) responsible annually for one million fatalities mostly among infants. Lipopolysaccharides (LPSs) are characteristic components of the outer membrane of the overwhelming majority of Gram\u2010negative bacteria. Since lipid A is essential for the viability of the Gram\u2010negative bacteria, it is subject to extensive chemical studies with new analytical techniques.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS<\/jats:title>\n                    <jats:p>Lipid A was released by mild acid hydrolysis from the lipopolysaccharide which was obtained via the phenol\/water extraction, purified and analyzed by matrix\u2010assisted laser desorption\/ionization time\u2010of\u2010flight mass spectrometry (MALDI\u2010TOF\u2010MS) and matrix\u2010assisted laser desorption\/ionization laser\u2010induced dissociation tandem mass spectrometry (MALDI\u2010LID\u2010MS\/MS).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      A detailed structural study of the whole lipid A obtained from\n                      <jats:italic>S. flexneri<\/jats:italic>\n                      variant X was carried out for the first time. Thus, we have shown that lipid A is a heterogeneous mixture having different numbers of acylated and phosphoethanolamine groups attached to the diglucosamine backbone. Furthermore, we found in the phenol phase an unusual hepta\u2010acylated lipid A species, although the abundance was very low.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS<\/jats:title>\n                    <jats:p>\n                      MALDI\u2010TOF\u2010MS allowed us to unravel the lipid A heterogeneity, which was not previously reported in\n                      <jats:italic>Sf<\/jats:italic>\n                      LPS. It is well known that slight variations of the chemical structure of lipid A may change its biological activity. Thus, the knowledge of the detailed chemical structure represents an essential step for further development of new preventive or therapeutically active compounds. Copyright \u00a9 2012 John Wiley &amp; Sons, Ltd.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.6306","type":"journal-article","created":{"date-parts":[[2012,7,23]],"date-time":"2012-07-23T04:35:21Z","timestamp":1343018121000},"page":"2011-2020","source":"Crossref","is-referenced-by-count":19,"title":["Characterization of lipid A profiles from\n                    <i>Shigella flexneri<\/i>\n                    variant X lipopolysaccharide"],"prefix":"10.1002","volume":"26","author":[{"given":"Adriana C.","family":"Casabuono","sequence":"first","affiliation":[{"name":"CIHIDECAR, Departamento de Qu\u00edmica Org\u00e1nica, Facultad de Cs Exactas y Naturales (1428) Universidad de Buenos Aires, Pabell\u00f3n II, Cdad. Universitaria, Bs. As.  Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia A.","family":"van der Ploeg","sequence":"additional","affiliation":[{"name":"INPB \u2013 Administraci\u00f3n Nacional de Laboratorios e Institutos de Salud, Dr. Carlos G. Malbran  Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9s D.","family":"Rog\u00e9","sequence":"additional","affiliation":[{"name":"INPB \u2013 Administraci\u00f3n Nacional de Laboratorios e Institutos de Salud, Dr. Carlos G. Malbran  Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susana B.","family":"Bruno","sequence":"additional","affiliation":[{"name":"INPB \u2013 Administraci\u00f3n Nacional de Laboratorios e Institutos de Salud, Dr. Carlos G. 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