{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T13:22:48Z","timestamp":1789132968373,"version":"build-2803163510"},"reference-count":52,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,1,10]],"date-time":"2013-01-10T00:00:00Z","timestamp":1357776000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2013,2,28]]},"abstract":"<jats:sec>\n                    <jats:title>RATIONALE<\/jats:title>\n                    <jats:p>Bromadiolone and brodifacoum, two common anticoagulant rodenticides, are involved in the majority of anticoagulant rodenticide poisoning cases in humans in China. Hair analysis can provide long\u2010term information on drug exposure. A method using liquid chromatography coupled with tandem mass spectrometry (LC\/MS\/MS) was developed and validated for the measurement of bromadiolone and brodifacoum in human hair.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS<\/jats:title>\n                    <jats:p>\n                      A 1\u2009mL aliquot of a phosphate buffer solution (pH 6.8) was added to 20\u2009mg of pulverized hair followed by ultrasonication and liquid\u2010liquid extraction. Liquid chromatography was performed using a C\n                      <jats:sub>18<\/jats:sub>\n                      column with a mobile phase gradient of ammonium acetate (10\u2009mM) and methanol. A tandem mass spectrometer in multiple reaction monitoring mode with a negative electrospray ionization source was employed for detection. Warfarin\u2010\n                      <jats:italic>d<\/jats:italic>\n                      <jats:sub>5<\/jats:sub>\n                      was used as an internal standard for both analytes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>The limits of detection (LODs) for bromadiolone and brodifacoum were 0.010 and 0.025\u2009ng\/mg, respectively. The calibration curves for both analytes were linear from 0.025 to 1\u2009ng\/mg. The accuracy ranged from 90.3 to 109.3%, and the intra\u2010day and inter\u2010day imprecisions were less than 15%.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS<\/jats:title>\n                    <jats:p>The established method was found effective when applied to the analyses of bromadiolone or brodifacoum in five cases, indicating that segmental hair analysis could be useful for clinical and forensic purposes by identifying the time of ingestion. Copyright \u00a9 2013 John Wiley &amp; Sons, Ltd.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.6477","type":"journal-article","created":{"date-parts":[[2013,1,16]],"date-time":"2013-01-16T00:13:40Z","timestamp":1358295220000},"page":"513-520","source":"Crossref","is-referenced-by-count":19,"title":["Determination of bromadiolone and brodifacoum in human hair by liquid chromatography\/tandem mass spectrometry and its application to poisoning cases"],"prefix":"10.1002","volume":"27","author":[{"given":"Lin","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Forensic Science, School of Basic Medical Sciences Fudan University  Shanghai 200032 P.R. China"},{"name":"Department of Forensic Toxicology, Institute of Forensic Science, Ministry of Justice, P. R. China Shanghai Key Laboratory of Forensic Medicine  Shanghai 200063 P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Forensic Toxicology, Institute of Forensic Science, Ministry of Justice, P. R. China Shanghai Key Laboratory of Forensic Medicine  Shanghai 200063 P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baohua","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Forensic Toxicology, Institute of Forensic Science, Ministry of Justice, P. R. China Shanghai Key Laboratory of Forensic Medicine  Shanghai 200063 P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Forensic Toxicology, Institute of Forensic Science, Ministry of Justice, P. R. China Shanghai Key Laboratory of Forensic Medicine  Shanghai 200063 P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Forensic Toxicology, Institute of Forensic Science, Ministry of Justice, P. R. China Shanghai Key Laboratory of Forensic Medicine  Shanghai 200063 P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Xiang","sequence":"additional","affiliation":[{"name":"Department of Forensic Toxicology, Institute of Forensic Science, Ministry of Justice, P. R. China Shanghai Key Laboratory of Forensic Medicine  Shanghai 200063 P.R. 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