{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T03:08:02Z","timestamp":1791083282200,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2013,5,7]],"date-time":"2013-05-07T00:00:00Z","timestamp":1367884800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2013,6,30]]},"abstract":"<jats:sec>\n                    <jats:title>RATIONALE<\/jats:title>\n                    <jats:p>Bioavailability of essential lipophilic micronutrients and carotenoids is of utmost interest for human health, as the consumption of these compounds may help alleviate major nutritional deficiencies, cardiovascular disease, and cancer. High\u2010performance liquid chromatography\/photo\u2010diode array detection (HPLC\u2010PDA) and high\u2010performance liquid chromatography\/tandem mass spectrometry (HPLC\/MS\/MS) were compared for the quantitative analysis of \u03b1\u2010 and \u03b2\u2010carotene, \u03b2\u2010cryptoxanthin, lutein, lycopene, \u03b1\u2010tocopherol, phylloquinone, and several retinyl esters from chylomicron\u2010containing triglyceride rich lipoprotein (TRL) fractions of human plasma obtained from two clinical trials.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS<\/jats:title>\n                    <jats:p>After selecting an efficient extraction method for the analytes, both the HPLC\/PDA and the HPLC\/MS\/MS methods were developed and several parameters validated using an HP 1200 series HPLC system interfaced with a HP 1200 series diode\u2010array detector (Agilent Technologies, Santa Clara, CA, USA) and a QTRAP 5500 (AB Sciex, Foster City, CA, USA) via an atmospheric pressure chemical ionization (APCI) probe operated in positive ion mode.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      For lycopene, \u03b1\u2010 and \u03b2\u2010carotene, HPLC\/MS\/MS was up to 37 times more sensitive than HPLC\u2010PDA. PDA detection was shown to be up to 8 times more sensitive for lutein. MS\/MS signals were enhanced by matrix components for lutein and \u03b2\u2010cryptoxanthin, as determined by referencing to the matrix\u2010independent PDA signal. In contrast, matrix suppression was observed for retinyl palmitate, \u03b1\u2010carotene, and \u03b2\u2010carotene. Both detectors showed similar suitability for \u03b1\u2010tocopherol, lycopene and retinyl palmitate (representing ~73% of total retinyl esters). MS\/MS exclusively allowed the quantitation of minor retinyl esters, phylloquinone, and (\n                      <jats:italic>Z<\/jats:italic>\n                      )\u2010lycopene isomers.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS<\/jats:title>\n                    <jats:p>HPLC\/MS\/MS was more sensitive than HPLC\u2010PDA for six of the eight analytes and represents a powerful tool for the analysis of chylomicron samples and potentially other biological samples of limited sample size. When internal standards are available for the target carotenoid, employing MS\/MS detection may reduce the necessary blood sample volume, which is particularly advantageous for minimizing risk and discomfort to human subjects during clinical studies. Copyright \u00a9 2013 John Wiley &amp; Sons, Ltd.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.6576","type":"journal-article","created":{"date-parts":[[2013,5,14]],"date-time":"2013-05-14T06:06:30Z","timestamp":1368511590000},"page":"1393-1402","source":"Crossref","is-referenced-by-count":53,"title":["Comparison of high\u2010performance liquid chromatography\/tandem mass spectrometry and high\u2010performance liquid chromatography\/photo\u2010diode array detection for the quantitation of carotenoids, retinyl esters, \u03b1\u2010tocopherol and phylloquinone in chylomicron\u2010rich fractions of human plasma"],"prefix":"10.1002","volume":"27","author":[{"given":"Rachel E.","family":"Kopec","sequence":"first","affiliation":[{"name":"Department of Food Science &amp; Technology The Ohio State University  Columbus OH USA"},{"name":"Department of Human Nutrition The Ohio State University  Columbus OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralf M.","family":"Schweiggert","sequence":"additional","affiliation":[{"name":"Department of Food Science &amp; Technology The Ohio State University  Columbus OH USA"},{"name":"Institute of Food Science and Biotechnology Hohenheim University  Stuttgart Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken M.","family":"Riedl","sequence":"additional","affiliation":[{"name":"Department of Food Science &amp; Technology The Ohio State University  Columbus OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reinhold","family":"Carle","sequence":"additional","affiliation":[{"name":"Institute of Food Science and Biotechnology Hohenheim University  Stuttgart Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven J.","family":"Schwartz","sequence":"additional","affiliation":[{"name":"Department of Food Science &amp; Technology The Ohio State University  Columbus OH USA"},{"name":"Department of Human Nutrition The Ohio State University  Columbus OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,5,7]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/133.7.2336"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/94.5.391"},{"key":"e_1_2_6_4_1","doi-asserted-by":"crossref","first-page":"975.21","DOI":"10.1096\/fasebj.25.1_supplement.975.21","article-title":"Brain levels of lutein (L) and zeaxanthin (Z) are related to cognitive function in centenarians","volume":"25","author":"Johnson E. 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