{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T14:57:36Z","timestamp":1761490656375,"version":"build-2065373602"},"reference-count":22,"publisher":"Wiley","issue":"19","license":[{"start":{"date-parts":[[2013,9,2]],"date-time":"2013-09-02T00:00:00Z","timestamp":1378080000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2013,10,15]]},"abstract":"\n RATIONALE<\/jats:title>\n Reported incidents of the use of nutritional supplements containing deer antler velvet by athletes has increased significantly in recent years. The supplements have been reported to contain insulin\u2010like growth factor\u20101 (IGF\u20101), which is a banned substance included on the World Anti\u2010Doping Agency (WADA) prohibited list. The presence of deer and human IGF\u20101 was tested in six commercially available supplements.<\/jats:p>\n <\/jats:sec>\n \n METHODS<\/jats:title>\n IGF\u20101 was extracted from the six deer antler velvet supplements using chloroform and acetonitrile precipitation methods. Ultra\u2010performance liquid chromatography\/tandem mass spectrometry (UPLC\/MS\/MS) methods were developed to measure intact IGF\u20101 protein and IGF\u20101 trypsin peptides using a triple quadrupole mass spectrometer. Five deer\u2010specific and five human\u2010specific multiple\u2010reaction monitoring (MRM) transitions for intact IGF\u20101were measured as well as six deer\u2010specific and seven human\u2010specific MRM transitions for an IGF\u20101 trypsin peptide.<\/jats:p>\n <\/jats:sec>\n \n RESULTS<\/jats:title>\n The peak area from each MRM transition was used to calculate the product ion ratios relative to the most abundant transition. Product ion ratios measured in the supplements were matched to ratios measured in purified protein standards. A match to human IGF\u20101 was identified for all the MRM transitions measured in four of the supplements tested.<\/jats:p>\n <\/jats:sec>\n \n CONCLUSIONS<\/jats:title>\n The presence of a pharmaceutical protein, human IGF\u20101, was confirmed in four commercially available products sold as all natural, nutritional supplements. These methods can be used to screen additional products to further prevent the illegal sale of adulterated supplements. Copyright \u00a9 2013 John Wiley & Sons, Ltd.<\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/rcm.6678","type":"journal-article","created":{"date-parts":[[2013,9,2]],"date-time":"2013-09-02T01:01:06Z","timestamp":1378083666000},"page":"2170-2178","source":"Crossref","is-referenced-by-count":12,"title":["Detection of human insulin\u2010like growth factor\u20101 in deer antler velvet supplements"],"prefix":"10.1002","volume":"27","author":[{"given":"Holly D.","family":"Cox","sequence":"first","affiliation":[{"name":"Sports Medicine Research and Testing Laboratory 560 Arapeen Dr., Suite 150A Salt Lake City UT 84108 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Eichner","sequence":"additional","affiliation":[{"name":"Sports Medicine Research and Testing Laboratory 560 Arapeen Dr., Suite 150A Salt Lake City UT 84108 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,9,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-010X(19980501)281:1<36::AID-JEZ6>3.0.CO;2-D"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ghir.2010.07.001"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.6144"},{"key":"e_1_2_7_5_1","unstructured":"WADA.The World Anti\u2010Doping Code: The 2013 Prohibited List International Standards 2013. Available:http:\/\/www.wada\u2010ama.org\/Documents\/World_Anti\u2010Doping_Program\/WADP\u2010Prohibited\u2010list\/2013\/WADA\u2010Prohibited\u2010List\u20102013\u2010EN.pdf."},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/08977190701773187"},{"key":"e_1_2_7_7_1","first-page":"80","article-title":"Health benefits of deer and elk velvet antler supplements: a systematic review of randomised controlled studies","volume":"125","author":"Gilbey A.","year":"2012","journal-title":"New Zealand Med. J."},{"key":"e_1_2_7_8_1","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1123\/ijsnem.13.3.251","article-title":"The effects of deer antler velvet extract or powder supplementation on aerobic power, erythropoiesis, and muscular strength and endurance characteristics","volume":"13","author":"Sleivert G.","year":"2003","journal-title":"Int. J. Sport Nutr. 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C.Parkin M.Thevis A.Thomas G. A.Woldemariam.Validation of an LC\u2010MS\/MS assay for quantification of serum insulin\u2010like growth factor\u20101. submitted."},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.135.1.223"},{"key":"e_1_2_7_19_1","unstructured":"WADA Technical Document \u2013 TD2010IDCR.Identification criteria for qualitative assays incorporating chromatography and mass spectrometry 2010. Available:http:\/\/www.wada\u2010ama.org\/Documents\/World_Anti\u2010Doping_Program\/WADP\u2010IS\u2010Laboratories\/Technical_Documents\/WADA_TD2010IDCRv1.0_Identification%20Criteria%20for%20Qualitative%20Assays_May%2008%202010_EN.doc.pdf."},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_2_7_21_1","unstructured":"Available:blast.ncbi.nlm.nih.gov."},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/js980080t"}],"container-title":["Rapid Communications in Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Frcm.6678","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rcm.6678","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T14:44:21Z","timestamp":1761489861000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/rcm.6678"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,9,2]]},"references-count":22,"journal-issue":{"issue":"19","published-print":{"date-parts":[[2013,10,15]]}},"alternative-id":["10.1002\/rcm.6678"],"URL":"https:\/\/doi.org\/10.1002\/rcm.6678","archive":["Portico"],"relation":{},"ISSN":["0951-4198","1097-0231"],"issn-type":[{"type":"print","value":"0951-4198"},{"type":"electronic","value":"1097-0231"}],"subject":[],"published":{"date-parts":[[2013,9,2]]}}}