{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T09:36:40Z","timestamp":1761471400111,"version":"build-2065373602"},"reference-count":22,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2013,10,7]],"date-time":"2013-10-07T00:00:00Z","timestamp":1381104000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2013,11,30]]},"abstract":"<jats:sec>\n                    <jats:title>RATIONALE<\/jats:title>\n                    <jats:p>Metabonomic studies use complex biological samples (blood plasma\/serum, tissues, etc.) that when analysed with high\u2010performance liquid chromatography\/mass spectrometry (HPLC\/MS) or nuclear magnetic resonance (NMR) generate profiles that may contain many thousands of features. These profiles can be difficult to interpret with the majority of the features contributing little to the study. As such there is an argument for the development of techniques that can simplify the problem by targeting particular classes of compounds.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS<\/jats:title>\n                    <jats:p>\n                      In this study ultra\u2010performance liquid chromatography\/inductively coupled plasma mass spectrometry (UPLC\/ICP\u2010MS) was used to profile tumour tissue and plasma samples for phosphorus\u2010 and sulfur\u2010containing metabolites. These samples were xenograft tumours (derived from breast, lung and colon cell lines) and plasma obtained from nude mice. Plasma was also obtained from non\u2010tumour\u2010bearing mice as a control. Due to isobaric interferences this method took advantage of the dynamic reaction cell within the ICP\u2010MS system to react the phosphorus and sulfur ions with oxygen. The PO\n                      <jats:sup>+<\/jats:sup>\n                      and SO\n                      <jats:sup>+<\/jats:sup>\n                      ions were then monitored free of interferences. The total phosphorus and sulfur within each sample was also recorded using flow injection ICP\u2010MS. A robust quality control system based on pooled sample replicate analysis was used throughout the study.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>Determination of the total phosphorus and sulfur content of each sample was sufficient in itself for statistical differentiation between the majority of the cell lines analysed. Subsequent reversed\u2010phase chromatographic profiling of the organic tumour and plasma extracts revealed the presence of a number of well\u2010retained phosphorus\u2010containing compounds that showed tumour\u2010specific profiles. Reversed\u2010phase profiling was not suitable for the sulfur\u2010containing compounds which eluted with the solvent front.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS<\/jats:title>\n                    <jats:p>This study has shown the potential use of UPLC\/ICP\u2010MS to differentiate between tumour cell lines, using both plasma and tumour tissue samples, based solely on metabolites that contain phosphorus or sulfur. Whilst further work is required to identify these compounds this methodology shows the ability of the described methods to provide targets for future biomarker discovery studies. Copyright \u00a9 2013 John Wiley &amp; Sons, Ltd.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.6722","type":"journal-article","created":{"date-parts":[[2013,10,11]],"date-time":"2013-10-11T21:08:01Z","timestamp":1381525681000},"page":"2539-2545","source":"Crossref","is-referenced-by-count":8,"title":["Phosphorus and sulfur metabonomic profiling of tissue and plasma obtained from tumour\u2010bearing mice using ultra\u2010performance liquid chromatography\/inductively coupled plasma mass spectrometry"],"prefix":"10.1002","volume":"27","author":[{"given":"David F.","family":"Thompson","sequence":"first","affiliation":[{"name":"School of Physical and Geographical Sciences Keele University  Keele Staffordshire ST5 5BG UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Filippos","family":"Michopoulos","sequence":"additional","affiliation":[{"name":"Oncology iMed AstraZeneca  Mereside, Alderley Park Macclesfield SK10 4TG UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher J.","family":"Smith","sequence":"additional","affiliation":[{"name":"Novartis Animal Health Australasia Pty Limited  Yarrandoo R&amp;D Centre, 245 Western Road Kemps Creek NSW 2178 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine J.","family":"Duckett","sequence":"additional","affiliation":[{"name":"Biomedical Research Centre Sheffield Hallam University  Owen Building Sheffield S1 1WB UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert W.","family":"Wilkinson","sequence":"additional","affiliation":[{"name":"MedImmune Limited  Aaron Klug Building, Granta Park Cambridge CB21 6GH UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philip","family":"Jarvis","sequence":"additional","affiliation":[{"name":"Discovery Statistics AstraZeneca  Mereside, Alderley Park Macclesfield SK10 4TG UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian D.","family":"Wilson","sequence":"additional","affiliation":[{"name":"Department of Surgery and Cancer Imperial College  Sir Alexander Fleming Building, Exhibition Rd, South Kensington London SW7 2AZ 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