{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T05:38:46Z","timestamp":1792647526581,"version":"4.5.0"},"reference-count":57,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,12,26]],"date-time":"2013-12-26T00:00:00Z","timestamp":1388016000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2014,2,28]]},"abstract":"<jats:sec>\n                    <jats:title>RATIONALE<\/jats:title>\n                    <jats:p>A sensitive and selective liquid chromatography\/mass spectrometry (LC\/MS) method is essential for quality control of synthetic oligonucleotides. However, researchers are still searching for improvements to ion\u2010pairing reagents for ion\u2010pairing reversed\u2010phase LC\/MS. This study performed a comprehensive comparison of six ion\u2010pairing reagents to determine their performance as mobile phase modifiers for oligonucleotide LC\/MS.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS<\/jats:title>\n                    <jats:p>\n                      The study was performed using a Waters ultra\u2010performance liquid chromatography (UPLC\u00ae) system coupled to a Waters LCT premier XE ESI\u2010TOF mass spectrometer by using a UPLC\u00ae OST column (2.1\u2009mm\u2009\u00d7\u2009100\u2009mm, 1.7\u00b5m). Buffer systems containing ion\u2010pairing reagents (triethylamine, tripropylamine, hexylamine,\n                      <jats:italic>N,N<\/jats:italic>\n                      \u2010dimethylbutylamine, dibutylamine,\n                      <jats:italic>N,N<\/jats:italic>\n                      \u2010diisopropylethylamine) and hexafluoro\u20102\u2010propanol were compared by measuring the adduct ion formation, chromatographic separation, and MS signal intensity of four oligonucleotides (10mer to 40mer). The effect of dissolution solvents on MS signal intensity and adduct ion formation was also investigated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Results showed that the type of dissolution solvent can have a signficiant impact on adduct ion formation with oligonucleotides. Results also showed that the maximum separation for small, medium and large oligonucleotides occured when using tripropylamine,\n                      <jats:italic>N,N<\/jats:italic>\n                      \u2010dimethylbutylamine, and dibutylamine, respectively. However, on average 15\u2009mM hexylamine and 50\u2009mM hexafluoro\u20102\u2010propanol provided the best chromtatographic performance (resolution values: 14.1\u2009\u00b1\u20090.34, 11.0\u2009\u00b1\u20090.17, and 6.4\u2009\u00b1\u20090.11 for the pairs of oligonucleotides T10 &amp; T15, T15 &amp; T25, and T25 &amp; T40, respectively (3 replicates)).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS<\/jats:title>\n                    <jats:p>The impact of dissolution solvent on the MS signal of oligonucleotides depends on the type of ion\u2010pairing reagent. Buffer combining 15\u2009mM hexylamine and 50\u2009mM hexafluoro\u20102\u2010propanol produced the highest overall performance for oligonucleotides (10mer to 40mer) with respect to chromatographic resolution and mass detection. Copyright \u00a9 2013 John Wiley &amp; Sons, Ltd.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.6773","type":"journal-article","created":{"date-parts":[[2013,12,27]],"date-time":"2013-12-27T01:51:16Z","timestamp":1388109076000},"page":"339-350","source":"Crossref","is-referenced-by-count":73,"title":["Comparing ion\u2010pairing reagents and sample dissolution solvents for ion\u2010pairing reversed\u2010phase liquid chromatography\/electrospray ionization mass spectrometry analysis of oligonucleotides"],"prefix":"10.1002","volume":"28","author":[{"given":"Lingzhi","family":"Gong","sequence":"first","affiliation":[{"name":"Chemistry Research Laboratory, Department of Chemistry University of Oxford  Mansfield Road Oxford OX1 3TA UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James S. 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