{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T12:02:29Z","timestamp":1777982549369,"version":"3.51.4"},"reference-count":26,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,2,7]],"date-time":"2014-02-07T00:00:00Z","timestamp":1391731200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2014,3,30]]},"abstract":"<jats:sec>\n                    <jats:title>RATIONALE<\/jats:title>\n                    <jats:p>Metachromatic leukodystrophy (MLD) is a genetic autosomal recessive disease caused by a deficiency in arylsulfatase A. Accumulated sulfatides can be detected in the urine and detection of sulfatiduria is a useful test for diagnosis and monitoring. To our knowledge, no studies have explored the accumulation of sulfatides in dried blood spots (DBSs). We developed an ultra\u2010performance liquid chromatography\/tandem mass spectrometry (UPLC\/MS\/MS) method for measuring sulfatides in DBSs from patients with MLD.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS<\/jats:title>\n                    <jats:p>DBSs were eluted with internal standard. After mixing and centrifugation, the organic layer was transferred to a 96\u2010well microplate and dried, then resuspended in methanol\/propanol solution. Samples were analyzed on an UPLC system. Total running time was 4\u2009min. Quantification was achieved by multiple reaction monitoring using a tandem mass spectrometer. We evaluated the precision, linearity, and ion suppression of the method and analyzed sulfatide concentrations in DBS specimens from MLD patients (n\u2009=\u20099), pseudodeficiency (PD) patient (n\u2009=\u20091), obligate heterozygotes (OH) (n\u2009=\u20092) and normal controls (n\u2009=\u2009124).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      In negative\u2010ion mode, sulfatides species subjected to collision\u2010induced dissociation readily fragment to produce an intense ion at\n                      <jats:italic>m\/z<\/jats:italic>\n                      96.8 (HSO\n                      <jats:sub>4<\/jats:sub>\n                      <jats:sup>\u2013<\/jats:sup>\n                      ). The precisions of low and high concentration controls ranged from 5.4 to 19.9%. The sulfatides produced linear responses. Molecular species of sulfatides were barely detected in DBSs from normal individuals and the PD\u2010OH group [mean (range), 0.07 (&lt;0.05\u20130.34) and 0.13 (&lt;0.05\u20130.22) \u00b5g\/mL, respectively]. In contrast, the DBSs from MLD patients showed a marked increase in several molecular species of sulfatide [mean (range), 2.02 (1.18\u20133.89) \u00b5g\/mL].\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS<\/jats:title>\n                    <jats:p>Simultaneous detection for sulfatides using UPLC\/MS\/MS can be successfully applied to DBS analysis. This method provides a fast and effective screening and monitoring tool for the diagnosis and treatment of MLD. Copyright \u00a9 2014 John Wiley &amp; Sons, Ltd.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.6823","type":"journal-article","created":{"date-parts":[[2014,2,7]],"date-time":"2014-02-07T01:33:01Z","timestamp":1391736781000},"page":"587-594","source":"Crossref","is-referenced-by-count":21,"title":["Ultra\u2010performance liquid chromatography\/tandem mass spectrometry for determination of sulfatides in dried blood spots from patients with metachromatic leukodystrophy"],"prefix":"10.1002","volume":"28","author":[{"given":"Minje","family":"Han","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine Seoul National University College of Medicine  Seoul Korea"},{"name":"Department of Laboratory Medicine Seoul National University Hospital  Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sun\u2010Hee","family":"Jun","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Seoul National University Bundang Hospital  Gyeonggi\u2010do Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sang Hoon","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Seoul National University College of Medicine  Seoul Korea"},{"name":"Department of Laboratory Medicine Seoul National University Hospital  Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyung\u2010Doo","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine &amp; Genetics, Samsung Medical Center Sungkyunkwan University School of Medicine  Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyoung Un","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Seoul National University College of Medicine  Seoul Korea"},{"name":"Department of Laboratory Medicine Seoul National University Bundang Hospital  Gyeonggi\u2010do Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junghan","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Seoul National University College of Medicine  Seoul Korea"},{"name":"Department of Laboratory Medicine Seoul National University Bundang Hospital  Gyeonggi\u2010do Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,2,7]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"3695","volume-title":"The Metabolic and Molecular Basis of Inherited Disease","author":"Figura K.","year":"2001"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymgme.2009.09.006"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0030-1253412"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004399900075"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.3343\/kjlm.2010.30.5.516"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1651-2227.2008.00648.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(59)90119-6"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1971.tb00562.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M500188-JLR200"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-9120(97)00142-2"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(87)90068-4"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddi126"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/dds086"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10545-011-9308-6"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0029074"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2011.178319"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2006.12.024"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwj122"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1999.4245"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1006\/mgme.2001.3165"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10719-007-9067-7"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.a.32712"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2013.06.027"},{"key":"e_1_2_7_25_1","unstructured":"W. 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