{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T03:04:11Z","timestamp":1778900651762,"version":"3.51.4"},"reference-count":53,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2014,4,8]],"date-time":"2014-04-08T00:00:00Z","timestamp":1396915200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100006031","name":"US-Israel Binational Agricultural Research and Development Fund","doi-asserted-by":"crossref","award":["US-4273-09"],"award-info":[{"award-number":["US-4273-09"]}],"id":[{"id":"10.13039\/100006031","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2014,5,30]]},"abstract":"<jats:sec>\n                    <jats:title>RATIONALE<\/jats:title>\n                    <jats:p>Monitoring of veterinary drug residues in foods is often conducted using liquid chromatography\/tandem mass spectrometry (LC\/MS\/MS). Results have high economic stakes for producers, but the ions monitored are usually selected due to signal intensities without structural interpretation. In this study, the ion transitions were characterized by high\u2010resolution mass spectrometry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS<\/jats:title>\n                    <jats:p>The 62 veterinary drugs from the LC\/MS\/MS method consisted of sulfonamides, \u03b2\u2010lactams, phenicols, macrolides, tetracyclines, fluoroquinolones, non\u2010steroidal anti\u2010inflammatory drugs (NSAIDs), and corticosteroids. They were individually infused into a quadrupole time\u2010of\u2010flight (Q\u2010TOF) mass spectrometer using electrospray ionization (ESI) operated in positive mode. The MS and collision\u2010induced dissociation (CID) MS\/MS spectra for each analyte were obtained for structural elucidation. The Q\u2010TOF instrument was calibrated to obtain a mass accuracy error &lt;5\u2009ppm for the MS and MS\/MS spectra.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>The use of high\u2010resolution ESI\u2010Q\u2010TOF\u2010MS for the generation of the MS\/MS product ions allowed for the determination of chemical formulae for the analytes, some of which led to new findings. Assigned structures were based on rational interpretation of the most stable possible products with comparison with the scientific literature. In difficult cases, isotopically labeled drugs or hydrogen\/deuterium (H\/D) exchange experiments were used to help confirm the structures of the product ions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS<\/jats:title>\n                    <jats:p>The use of ESI\u2010Q\u2010TOF\u2010MS in this study has allowed structure elucidation of 186 MS\/MS product ions previously selected for the LC\/MS\/MS analysis of 62 veterinary drugs. This serves to reduce the chances of false positives and negatives in the monitoring program, and provides justification and defense in regulatory enforcement actions. Published in 2014. This article is a US Government work and is in the public domain in the USA.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.6871","type":"journal-article","created":{"date-parts":[[2014,4,8]],"date-time":"2014-04-08T01:06:18Z","timestamp":1396919178000},"page":"1061-1081","source":"Crossref","is-referenced-by-count":27,"title":["Structural characterization of product ions by electrospray ionization and quadrupole time\u2010of\u2010flight mass spectrometry to support regulatory analysis of veterinary drug residues in foods"],"prefix":"10.1002","volume":"28","author":[{"given":"Luc\u00eda","family":"Geis\u2010Asteggiante","sequence":"first","affiliation":[{"name":"US Department of Agriculture, Agricultural Research Service Eastern Regional Research Center  600 East Mermaid Lane Wyndmoor PA 19038 USA"},{"name":"C\u00e1tedra de Farmacognosia y Productos Naturales, DQO, Facultad de Qu\u00edmica UdelaR  General Flores 2124 12800 Montevideo Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberto","family":"Nu\u00f1ez","sequence":"additional","affiliation":[{"name":"US Department of Agriculture, Agricultural Research Service Eastern Regional Research Center  600 East Mermaid Lane Wyndmoor PA 19038 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven J.","family":"Lehotay","sequence":"additional","affiliation":[{"name":"US Department of Agriculture, Agricultural Research Service Eastern Regional Research Center  600 East Mermaid Lane Wyndmoor PA 19038 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan R.","family":"Lightfield","sequence":"additional","affiliation":[{"name":"US Department of Agriculture, Agricultural Research Service Eastern Regional Research Center  600 East Mermaid Lane Wyndmoor PA 19038 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,4,8]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"U.S. Food and Drug Administration U.S. Department of Agriculture.A Description of the U.S. Food Safety System March 3 2000. 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Available:www.fsis.usda.gov\/wps\/portal\/fsis\/topics\/data\u2010collection\u2010and\u2010reports\/chemistry\/red\u2010books\/archive."},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.1363"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2012.08.020"},{"key":"e_1_2_7_8_1","unstructured":"U.S. Department of Agriculture.Food Safety and Inspection Service.Chemistry Laboratory Guidebook: Screening and Confirmation of Animal Drug Residues by UHPLC\u2010MS\u2010MS. April 15 2013. 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