{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T07:43:13Z","timestamp":1775979793466,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T00:00:00Z","timestamp":1771372800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T00:00:00Z","timestamp":1771372800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2026,5,30]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The rising incidence of Salmonella\u2010induced diarrhea highlights the critical need for judicious antibiotic use to ensure effective treatment outcomes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This study presents an innovative methodology for evaluating the susceptibility of Salmonella to ceftriaxone and azithromycin through the application of matrix\u2010assisted laser desorption\/ionization time\u2010of\u2010flight mass spectrometry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The proposed method requires only 2\u20133 individual Salmonella colonies and yields results within approximately 1 hour, assessing susceptibility to ceftriaxone (70 mg\/L) and azithromycin (25 mg\/L). In comparison to the Kirby\u2010Bauer method, this technique exhibits a sensitivity of 98.64% and a specificity of 84.38%.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The method developed in this study is both straightforward and user\u2010friendly, making it suitable for deployment in major laboratories equipped with time\u2010of\u2010flight mass spectrometry. It offers a preliminary assessment of Salmonella susceptibility to ceftriaxone and azithromycin, thereby enhancing the accuracy of clinical interventions for Salmonella infections.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.70053","type":"journal-article","created":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T08:17:04Z","timestamp":1771402624000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Sensitive Detection of Salmonella Susceptibility to Ceftriaxone and Azithromycin Using Matrix\u2010Assisted Laser Desorption\/Ionization Time\u2010of\u2010Flight Mass Spectrometry"],"prefix":"10.1002","volume":"40","author":[{"given":"Guohao","family":"Zhan","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory DongGuan SongShan Lake Tungwah Hospital  Dongguan Guangdong China"},{"name":"Dongguan Key Laboratory of Medical Molecular Biology  Dongguan Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shujin","family":"Xie","sequence":"additional","affiliation":[{"name":"Dongguan Key Laboratory of Medical Molecular Biology  Dongguan Guangdong China"},{"name":"Department of Clinical Laboratory DongGuan Tungwah Hospital  Dongguan Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinyu","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Medical Technology Guangdong Medical University  Dongguan Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingyi","family":"Liang","sequence":"additional","affiliation":[{"name":"School of Medical Technology Guangdong Medical University  Dongguan Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaojun","family":"Huang","sequence":"additional","affiliation":[{"name":"Dongguan Key Laboratory of Medical Molecular Biology  Dongguan Guangdong China"},{"name":"Department of Clinical Laboratory DongGuan Tungwah Hospital  Dongguan Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory DongGuan SongShan Lake Tungwah Hospital  Dongguan Guangdong China"},{"name":"Dongguan Key Laboratory of Medical Molecular Biology  Dongguan Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fei","family":"Lv","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory DongGuan SongShan Lake Tungwah Hospital  Dongguan Guangdong China"},{"name":"Dongguan Key Laboratory of Medical Molecular Biology  Dongguan Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-5403-9062","authenticated-orcid":false,"given":"Zhusheng","family":"Guo","sequence":"additional","affiliation":[{"name":"Dongguan Key Laboratory of Medical Molecular Biology  Dongguan Guangdong China"},{"name":"Department of Clinical Laboratory DongGuan Tungwah Hospital  Dongguan Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,18]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.764787"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.01268"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2023.0094"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfp.2024.100219"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2025.106508"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02849-15"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9020260"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2013.02.008"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12941-024-00729-9"},{"key":"e_1_2_11_11_1","volume-title":"Performance Standards for Antimicrobial Susceptibility Testing Guideline M100","author":"CLSI","year":"2024"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.psj.2024.104430"},{"issue":"8","key":"e_1_2_11_13_1","first-page":"750","article-title":"Surveillance of Salmonella Infection and Antimicrobial Resistance in Foodborne Diseases among Children in Wuhan, 2017\u20132022","volume":"40","author":"Nie Li D. 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