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The method was fully validated according to FDA standards.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>All analytes showed good linearity over the concentration range of 0.19\u201319.53\u2009ng\/mL, with lower limits of quantification (LLOQs) ranging from 0.19 to 19.53\u2009ng\/mL. Accuracy (relative bias) and precision (relative standard deviation) were within \u00b115%. Matrix effects were evaluated (85.7%\u2013114.3%), and extraction efficiencies were found to be satisfactory (89.1%\u2013108.9%). Compared with other LC\u2010MS\u2010based techniques, this method effectively eliminates interference from endogenous substances by utilizing SPE columns.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The proposed method exhibited excellent performance in terms of linearity, precision, accuracy, stability, and matrix effects, demonstrating superior practicality and reliability for the analysis of complex biological samples.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.70063","type":"journal-article","created":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T10:32:27Z","timestamp":1773657147000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Simultaneous Quantification of Twelve Endogenous HPA Axis Compounds by Protein Precipitation and HILIC\u2010UPLC\u2013MS\/MS in a Mouse Model 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