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However, the active substance of LGZG and the specific mechanism remain unclear. This study aims to identify the bioactive components of LGZG and subsequently elucidate the underlying therapeutic mechanisms against OSA based on mass spectrometry analysis, network pharmacology, transcriptomics, and experimental verification.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Ultra\u2010high\u2010performance liquid chromatography\u2010high\u2010resolution mass spectrometry (UHPLC\u2013HRMS) was used to identify the main ingredients of LGZG. The OSA animal model was induced by chronic intermittent hypoxia (CIH) for 5\u2009weeks in C57BL\/6 mice. Transcriptome sequencing and network pharmacology were used to analyze potential mechanisms, which were further validated by molecular docking. HE staining was used for detecting lung inflammation. Immunohistochemistry (IHC), ELISA, and Western blot were employed to investigate protein expression, while quantitative real\u2010time PCR (RT\u2010qPCR) was used to determine gene expressions. Tissue reactive oxygen species (ROS) levels were measured by the DCFH\u2010DA probe method.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>LGZG inhibited CIH\u2010induced pulmonary inflammatory infiltration, protein concentration in bronchoalveolar lavage fluid (BALF), and suppressed IL\u201017 and IL\u20101\u03b2 gene expression. UHPLC\u2013HRMS identified 482 compounds in the LGZG aqueous decoction. Network pharmacology analysis revealed that IL\u20106 and HIF\u20101\u03b1 pathway were the major targets. Subsequently, transcriptomics analysis revealed that LGZG affected functions associated with ROS production and polymorphonuclear cells. LGZG suppressed the CIH\u2010induced expression of neutrophil elastase and reduced MPO production in BALF. Furthermore, LGZG inhibited the IL\u20106 expression and secretion, and reduced CIH\u2010induced ROS production. LGZG inhibited the CIH\u2010induced activation of HIF\u20101\u03b1 pathway. Moreover, molecular docking identified compounds in LGZG that could directly interact with the core targets IL\u20106, MPO, CYBB, and HIF\u20101\u03b1.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>LGZG alleviates CIH\u2010induced pulmonary inflammation, neutrophil infiltration, and IL\u20106 secretion in mice. These effects are associated with the suppression of ROS production and inhibition of the HIF\u20101\u03b1 signaling pathway.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.70064","type":"journal-article","created":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T05:04:44Z","timestamp":1774242284000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Lingguizhugan Decoction Alleviates Lung Inflammatory in Obstructive Sleep Apnea by Modulating ROS and HIF\u20101\u03b1 Signaling Pathway Based on UHPLC\u2013MS, Network Pharmacology, Transcriptomics, and Experimental Verification"],"prefix":"10.1002","volume":"40","author":[{"given":"Jinjin","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Respiratory and Critical Care The Second Affiliated Hospital of Soochow University  Soochow China"},{"name":"Department of Respiratory Medicine, Putuo Hospital Shanghai University of Traditional 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