{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T09:28:45Z","timestamp":1777627725919,"version":"3.51.4"},"reference-count":29,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T00:00:00Z","timestamp":1774915200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T00:00:00Z","timestamp":1774915200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2026,6,30]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Taiwan produces high\u2010value specialty coffee on a limited scale, whereas Brazil is the largest global supplier and a major source of imports to Taiwan. For food authenticity, differentiation of roasted coffee from these origins is important. However, few metabolomics\u2010based traceability studies have yet been conducted on Taiwan\u2010roasted coffee.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      In this study, metabolic profiling was applied to evaluate origin\u2010related chemical signatures in\n                      <jats:italic>Arabica<\/jats:italic>\n                      roasted coffee beans from Taiwan and Brazil. An untargeted metabolomic workflow of integrating full scan and data\u2010dependent acquisition (IFSDDA) was implemented using UHPLC\u2010QTOF\u2010MS. Roasted coffee beans were extracted using optimized 50% ethanol, profiled by LC\u2010MS\/MS, and analyzed using multivariate statistics, ROC analysis, and spectral\u2010library\u2013based metabolite annotation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Multivariate analyses revealed a clear separation of Taiwanese and Brazilian roasted coffee samples. Among 26 annotated metabolites, four compounds showed the highest discriminatory power (VIP &gt;\u20091.0), including 4\u2010(1H\u2010indol\u20103\u2010yl)butan\u20102\u2010one, citrulline, 7\u2010methanesulfinylheptanenitrile, and phytosphingosine. The four\u2010potential markers achieved excellent classification performance (AUC &gt;\u20090.98), reflecting consistent origin\u2010associated patterns.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This study demonstrated that untargeted metabolic profiling can effectively distinguish roasted coffee from Taiwan and Brazil. Such profiling provided an approach for food safety surveillance by capturing chemical signatures linked to regional production environments and agricultural inputs. The annotated markers offer a practical framework for monitoring both imported and domestic roasted coffee.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.70070","type":"journal-article","created":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T01:29:46Z","timestamp":1775006986000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative Metabolomic Profiling of Roasted Coffee Beans From Taiwan and Brazil"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0858-4349","authenticated-orcid":false,"given":"Han\u2010Ju","family":"Chien","sequence":"first","affiliation":[{"name":"Department of Biochemical Science and Technology National Chiayi University  Chiayi Taiwan"},{"name":"The Education and Research Center of Intelligent Technology for Food and Agriculture National Chiayi University  Chiayi Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-9242-8417","authenticated-orcid":false,"given":"Jyun\u2010Cih","family":"Jian","sequence":"additional","affiliation":[{"name":"Department of Biochemical Science and Technology National Chiayi University  Chiayi Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7133-8266","authenticated-orcid":false,"given":"Chien\u2010Chen","family":"Lai","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology National Chung Hsing University  Taichung Taiwan"},{"name":"Advanced Plant and Food Crop Biotechnology Center National Chung Hsing University  Taichung Taiwan"},{"name":"Graduate Institute of Chinese Medical Science China Medical University  Taichung Taiwan"},{"name":"Doctoral Program in Translational Medicine National Chung Hsing University  Taichung Taiwan"},{"name":"Rong Hsing Translational Medicine Research Center National Chung Hsing University  Taichung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,31]]},"reference":[{"key":"e_1_2_10_2_1","unstructured":"M.Bozzola S.Charles T.Ferretti et\u00a0al. \u201cThe Coffee Guide \u201d (2021)."},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2018.08.061"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2020.128266"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.5219\/1062"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo12040357"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471142727.mb3002s98"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216\u2010018\u20101405\u2010z"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1155\/2023\/6696808"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2024.141683"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.33176\/aacb\u201019\u201000023"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.9b05135"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.7042.1"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talo.2025.100597"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41597-024-03822-y"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11306-007-0070-6"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/fsn3.1904"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/fsn3.2849"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/fermentation10080435"},{"key":"e_1_2_10_20_1","doi-asserted-by":"crossref","unstructured":"O. R.PortilloandA. C.Ar\u00e9valo \u201cThe Coffee Bean Nitrogenous Compounds \u201d A Critical Review of Contemporary Scientific Literature (2024) https:\/\/doi.org\/10.18687\/laccei2024.1.1.501.","DOI":"10.18687\/LACCEI2024.1.1.501"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2023.113814"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2023.e21402"},{"key":"e_1_2_10_23_1","doi-asserted-by":"crossref","unstructured":"M. M.Art\u00eancio A. L. L.Cassago R. K.Silva J. d. M. E.Giraldi andF. B. D.Costa \u201cUntargeted Metabolomic Approach Based on UHPL\u2010ESI\u2010HRMS To Investigate Metabolic Profiles of Different Coffea Species and Terroir\u201d (2023) https:\/\/doi.org\/10.21203\/rs.3.rs\u20102828021\/v1.","DOI":"10.21203\/rs.3.rs-2828021\/v1"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fochx.2025.102808"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26133856"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(01)03123-4"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2020.00512"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.010261"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-05083-4"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-016-2545-z"}],"container-title":["Rapid Communications in Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rcm.70070","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/rcm.70070","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rcm.70070","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T01:17:51Z","timestamp":1777425471000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/rcm.70070"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,31]]},"references-count":29,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2026,6,30]]}},"alternative-id":["10.1002\/rcm.70070"],"URL":"https:\/\/doi.org\/10.1002\/rcm.70070","archive":["Portico"],"relation":{},"ISSN":["0951-4198","1097-0231"],"issn-type":[{"value":"0951-4198","type":"print"},{"value":"1097-0231","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,31]]},"assertion":[{"value":"2026-01-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-31","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70070"}}