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Oxidative stress initiates lipid peroxidation, generating a spectrum of oxidised phosphatidylcholines (oxPCs). OxPCs are commonly identified by their positional and isomeric variations. However, their structural diversity and chemical reactivity make their identification challenging. Oligomeric proanthocyanidins (OPCs) are bioactive metabolites of\n                      <jats:italic>Rhodiola crenulata<\/jats:italic>\n                      , well\u2010known for their potential therapeutic antioxidant and anti\u2010inflammatory effects. However, the study of alterations in oxPCs profiles associated with AHI and their regulation on\n                      <jats:italic>Rhodiola crenulata<\/jats:italic>\n                      OPC pre\u2010treatment remains unexplored.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      An induced carbon tetrachloride (CCl\n                      <jats:sub>4<\/jats:sub>\n                      ) AHI mouse model was constructed, and hepatic damage was assessed using biochemical and histochemical assays of the serum. An inbuilt library was developed utilising electron ionisation mass spectroscopy coupled with data\u2010dependent scanning (ESI\u2010DD\/MS) to characterise the oxPC. Multivariate data analysis and high\u2010resolution MS\/MS fragmentation patterns enabled the identification and quantification of potential oxPC biomarkers of AHI.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The targeted lipidomics workflow enabled the detection and quantification of 45 oxPCs, including 11 full\u2010chain oxPCs, and 34 truncated aldehyde\/carboxylate\u2010containing oxPC subclasses. Semi\u2010quantification of the oxPCs demonstrated an extensive accumulation of truncated oxPCs in the model, whereas OPC pre\u2010treatment significantly suppressed the oxPC formation. The antioxidant biomarkers and hepatocellular morphology were improved, indicating the hepatoprotective effects of OPC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study reveals that a comprehensive oxPC species profiling provides sensitive biomarkers of lipid peroxidation. By mitigating oxidative stress, restoring antioxidant enzymes and suppressing oxPCs formation, OPC demonstrates hepatoprotective effects.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.70088","type":"journal-article","created":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T12:54:11Z","timestamp":1778676851000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Rapid Lipidomics Analysis of Oxidised Phosphatidylcholines in Carbon Tetrachloride\u2010Induced Acute Hepatic Injury in Mice and Their Regulation by\n                    <i>Rhodiola crenulata<\/i>\n                    Oligomeric Proanthocyanidins"],"prefix":"10.1002","volume":"40","author":[{"given":"Charity Ngina","family":"Mwangi","sequence":"first","affiliation":[{"name":"Institute of Traditional Chinese Medicine Tianjin University of Traditional Chinese Medicine  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Jia","sequence":"additional","affiliation":[{"name":"Institute of Traditional Chinese Medicine Tianjin University of Traditional Chinese Medicine  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingrui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Traditional Chinese Medicine Tianjin University of Traditional Chinese Medicine  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-8355-447X","authenticated-orcid":false,"given":"Miaomiao","family":"Jiang","sequence":"additional","affiliation":[{"name":"Institute of Traditional Chinese Medicine Tianjin University of Traditional Chinese Medicine  Tianjin China"},{"name":"Haihe Laboratory of Modern Chinese Medicine  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,13]]},"reference":[{"key":"e_1_2_14_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010024\u201002072\u2010z"},{"key":"e_1_2_14_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2024.155663"},{"key":"e_1_2_14_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2024.06.016"},{"key":"e_1_2_14_5_1","doi-asserted-by":"publisher","DOI":"10.4103\/jpbs.jpbs_568_25"},{"key":"e_1_2_14_6_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2025.13471"},{"key":"e_1_2_14_7_1","volume-title":"StatPearls","author":"Moriles K. 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