{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T03:03:53Z","timestamp":1791083033823,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T00:00:00Z","timestamp":1777334400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T00:00:00Z","timestamp":1777334400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2026,8,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Migraine is a debilitating neurological disorder characterized by headaches, autonomic changes, and heightened sensitivity to stimuli. Traditional Chinese medicine, particularly the Chuanxiong\u2013Baizhi (CX\u2010BZ) herb pair, has shown clinical efficacy in migraine management, but its underlying mechanisms remain unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A migraine model was established by subcutaneous NTG injection. Sixty Sprague\u2013Dawley rats were randomly divided into control, model, positive, and low\u2010, medium\u2010, and high\u2010dose CX\u2010BZ groups. Behavioral tests were used to assess writhing behavior and thermal pain thresholds. Histopathological examination and enzyme\u2010linked immunosorbent assays were performed to evaluate neuronal damage and inflammatory cytokines. Metabolic alterations were analyzed using untargeted metabolomics based on UHPLC\u2010Orbitrap\u2010MS, followed by targeted metabolomics validation with UPLC\u2013QQQ\u2013MS.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>CX\u2010BZ pretreatment significantly reduced migraine\u2010like behaviors, including writhing frequency, and increased pain thresholds in NTG\u2010induced rats. It also lowered calcitonin gene\u2010related peptide (CGRP) and tumor necrosis factor\u2010\u03b1 (TNF\u2010\u03b1) levels in serum and the medulla. Metabolomic profiling identified distinct metabolic alterations between the model and CX\u2010BZ\u2010treated groups, mainly involving amino acid metabolism and the tricarboxylic acid (TCA) cycle. Targeted metabolomics confirmed the regulation of key metabolites within these pathways.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>CX\u2010BZ exerts preventive effects against migraine by modulating inflammatory responses and restoring metabolic homeostasis, particularly through the regulation of amino acid metabolism and the TCA cycle. These findings provide new insights into the multi\u2010target protective mechanisms of CX\u2010BZ in migraine prevention.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.70090","type":"journal-article","created":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T03:52:53Z","timestamp":1777434773000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Untargeted and Targeted Metabolomics Reveal the Regulatory Mechanisms of the Chuanxiong\u2013Baizhi Herb Pair in Treating Migraine"],"prefix":"10.1002","volume":"40","author":[{"given":"Yu","family":"Wang","sequence":"first","affiliation":[{"name":"Experiment Center for Science and Technology Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingwen","family":"Feng","sequence":"additional","affiliation":[{"name":"Experiment Center for Science and Technology Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5884-6023","authenticated-orcid":false,"given":"Rong","family":"Shi","sequence":"additional","affiliation":[{"name":"Experiment Center for Science and Technology Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ximei","family":"Yang","sequence":"additional","affiliation":[{"name":"Experiment Center for Science and Technology Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huishan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Experiment Center for Science and Technology Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lianrong","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Cancer Hospital Affiliated to Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengtian","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Pharmacy Jilin University  Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinhua","family":"Liu","sequence":"additional","affiliation":[{"name":"Experiment Center for Science and Technology Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingyun","family":"Pan","sequence":"additional","affiliation":[{"name":"Experiment Center for Science and Technology Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,28]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neubiorev.2024.105536"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40001\u2010024\u201001682\u20101"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1155\/2024\/1377143"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s10194\u2010019\u20101066\u20100"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572\u2010021\u201000328\u20104"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00281\u2010018\u20100676\u2010y"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s10194\u2010021\u201001229\u20103"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.21037\/atm\u201021\u20103423"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2019.03.026"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.3109\/13880209.2011.576346"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.805984"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2009.02.011"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.832673"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2023.113723"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2021.118971"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2016.11.025"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2019.112385"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2023.1135264"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.4625"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2016.01.033"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2012.07.033"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.19540\/j.cnki.cjcmm.20180115.004"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2018.09.232"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1039\/c9ra06267j"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2017.11.203"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2019.8201"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19040954"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.3945\/an.115.011221"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726\u2010012\u20101330\u2010y"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu15184023"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13311\u2010018\u20100616\u20105"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.13422\/j.cnki.syfjx.2011.14.072"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells10102756"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12883\u2010018\u20101047\u2010y"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.920201"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.2147\/JPR.S329280"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25021302"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1177\/0333102416640501"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2017.07.011"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2019.113018"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1086\/321282"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5454.848"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1175689"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.08.064"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1080\/03602532.2022.2162075"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2023.155266"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0117232"}],"container-title":["Rapid Communications in Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rcm.70090","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/rcm.70090","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rcm.70090","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T16:52:12Z","timestamp":1782924732000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/rcm.70090"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,28]]},"references-count":47,"journal-issue":{"issue":"15","published-print":{"date-parts":[[2026,8,15]]}},"alternative-id":["10.1002\/rcm.70090"],"URL":"https:\/\/doi.org\/10.1002\/rcm.70090","archive":["Portico"],"relation":{},"ISSN":["0951-4198","1097-0231"],"issn-type":[{"value":"0951-4198","type":"print"},{"value":"1097-0231","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,28]]},"assertion":[{"value":"2025-11-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70090"}}