{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T15:21:45Z","timestamp":1783092105861,"version":"3.54.6"},"reference-count":45,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T00:00:00Z","timestamp":1779840000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T00:00:00Z","timestamp":1779840000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2026,8,30]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study aimed to investigate the therapeutic mechanisms of CY in treating UC using an integrated metabolomics and proteomics approach.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A murine UC model was induced using dextran sulfate sodium (DSS). The therapeutic efficacy of CY was evaluated via histological examination (H&amp;E staining) of colon tissues to assess inflammation, epithelial necrosis, and crypt abscesses. Serum and colon tissues were collected for ELISA, immunofluorescence, Western blotting, metabolomics, and proteomics analyses. Additionally, an in\u00a0vitro intestinal epithelial barrier model was established using LPS\u2010induced Caco\u20102 cells. Cell viability, barrier function (TEER and FITC\u2010Dextran permeability), cell damage (LDH), and inflammatory cytokine levels (IL\u20106 and IL\u20101\u03b2) were measured. The expression and distribution of tight junction proteins (ZO\u20101 and occludin) and signaling proteins (FAK, p\u2010FAK, paxillin, and p\u2010paxillin) were analyzed via immunofluorescence and Western blotting.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>CY treatment significantly reduced the Disease Activity Index (DAI) and suppressed the levels of pro\u2010inflammatory cytokines (IL\u20106, IL\u20101\u03b2, and TNF\u2010\u03b1) in UC mice. Proteomic analysis identified Caveolin\u20101 (CAV1) and the focal adhesion pathway as key therapeutic targets and signaling pathways. Metabolomic profiling revealed 17 endogenous metabolites, highlighting tryptophan metabolism and butyrate metabolism as critical pathways. Furthermore, CY intervention modulated arachidonic acid and linoleic acid metabolism. Mechanistically, CY upregulated the expression of CAV1 and FAK while downregulating AKT and paxillin phosphorylation. In\u00a0vitro experiments demonstrated that CY\u2010containing serum restored the barrier function of LPS\u2010induced Caco\u20102 cells by regulating FAK\/paxillin phosphorylation, upregulating tight junction proteins, and mitigating inflammatory responses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>CY involvement in colonic mucosal healing attenuates inflammation and enhances intestinal barrier function in DSS\u2010induced colitis, likely through the modulation of the CAV1\/FA axis. These findings provide a theoretical basis for the clinical application of CY and offer novel insights for the development of therapeutic agents for UC.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.70106","type":"journal-article","created":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T11:50:47Z","timestamp":1779882647000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Chishizhi Yuyuliang Decoction Ameliorates Ulcerative Colitis in Mice by Promoting Colonic Epithelial Mucosal Healing via the CAV1\/FA Axis"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-0183-9805","authenticated-orcid":false,"given":"Xing","family":"Hong","sequence":"first","affiliation":[{"name":"College of Pharmacy Hubei University of Chinese Medicine  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxin","family":"Wen","sequence":"additional","affiliation":[{"name":"College of Pharmacy Hubei University of Chinese Medicine  Wuhan China"},{"name":"Public Inspection and Testing Center of Xianning  Xianning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-3352-0342","authenticated-orcid":false,"given":"Benjiang","family":"Xiao","sequence":"additional","affiliation":[{"name":"College of Pharmacy Hubei University of Chinese Medicine  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-9038-0281","authenticated-orcid":false,"given":"Qi","family":"Jiang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Traditional Chinese Medicine Resources and Chemistry of Hubei Province  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Traditional Chinese Medicine Resources and Chemistry of Hubei Province  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengyu","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Traditional Chinese Medicine Resources and Chemistry of Hubei Province  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Qian","sequence":"additional","affiliation":[{"name":"Key Laboratory of Traditional Chinese Medicine Resources and Chemistry of Hubei Province  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiong","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Traditional Chinese Medicine Resources and Chemistry of Hubei Province  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Traditional Chinese Medicine Resources and Chemistry of Hubei Province  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Pharmacy Hubei University of Chinese Medicine  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuang","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Pharmacy Hubei University of Chinese Medicine  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lintao","family":"Han","sequence":"additional","affiliation":[{"name":"Key Laboratory of Traditional Chinese Medicine Resources and Chemistry of Hubei Province  Wuhan China"},{"name":"Faculty of Pharmacy Hubei University of Chinese Medicine  Wuhan China"},{"name":"School of Basic Medical Science Hubei University of Chinese Medicine  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,27]]},"reference":[{"issue":"22","key":"e_1_2_11_2_1","first-page":"2810","article-title":"Research Progress on Metabonomics of Traditional Chinese Medicine in the Treatment of Ulcerative Colitis","volume":"34","author":"Lin B.","year":"2023","journal-title":"China Pharmacy"},{"key":"e_1_2_11_3_1","unstructured":"CLe Berre SHonap andLPeyrin\u2010Biroulet \u201cUlcerative Colitis \u201d (1474\u2010547X (Electronic))."},{"key":"e_1_2_11_4_1","unstructured":"B A\u2010OZhou QYu XJiang et\u00a0al. \u201cAssociation Between Inflammatory Bowel Disease and Risk of Incident Prostate Cancer: A Systematic Review and Meta\u2010Analysis of Cohort Studies \u201d (1432\u20131262 (Electronic))."},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12937\u2010025\u201001075\u2010y"},{"key":"e_1_2_11_6_1","unstructured":"J DFeuerstein K LIsaacs YSchneider et\u00a0al. \u201cAGA Clinical Practice Guidelines on the Management of Moderate to Severe Ulcerative Colitis \u201d (1528\u20130012 (Electronic))."},{"key":"e_1_2_11_7_1","unstructured":"G. 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