{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T16:45:34Z","timestamp":1790181934950,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2026,6,14]],"date-time":"2026-06-14T00:00:00Z","timestamp":1781395200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,14]],"date-time":"2026-06-14T00:00:00Z","timestamp":1781395200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2026,9,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Hyperuricemia (HUA) is a metabolic disorder commonly accompanied by oxidative stress and inflammatory responses, which contribute to the progression of kidney and liver injury. Clematichinenoside AR (C\u2010AR), a natural triterpenoid saponin, has been reported to regulate metabolism and exert anti\u2010inflammatory and antioxidant activities. However, its therapeutic potential and underlying mechanisms in HUA remain unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The anti\u2010hyperuricemic effect of C\u2010AR was evaluated in a mouse model of HUA. Biochemical assays and histopathological analyses were performed to assess uric acid metabolism and organ injury. The expression of inflammation\u2010, oxidative stress\u2010, and urate transport\u2010related factors was determined by RT\u2010qPCR and Western blotting. Untargeted serum metabolomics was conducted using UPLC\u2010Q\u2010TOF\u2010MS\/MS to investigate metabolic alterations associated with C\u2010AR treatment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>C\u2010AR significantly reduced serum uric acid levels, inhibited xanthine oxidase activity, and alleviated uric acid\u2010induced kidney and liver injury. It suppressed inflammatory responses by decreasing TNF\u2010\u03b1, IL\u20106, and IL\u20101\u03b2 levels and attenuated oxidative stress by reducing renal malondialdehyde levels. Furthermore, C\u2010AR upregulated ABCG2, NRF2, and HO\u20101 expression while downregulating NLRP3 and Caspase\u20101. Untargeted metabolomics revealed that C\u2010AR predominantly regulated purine metabolism and restored metabolic disturbances associated with HUA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>C\u2010AR effectively ameliorates hyperuricemia by regulating uric acid metabolism, suppressing inflammation, and alleviating oxidative stress. These effects are associated with modulation of the NRF2\/HO\u20101 signaling pathway and purine metabolism, supporting the potential development of C\u2010AR as a natural therapeutic agent for hyperuricemia.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.70109","type":"journal-article","created":{"date-parts":[[2026,6,14]],"date-time":"2026-06-14T13:12:15Z","timestamp":1781442735000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Exploration of the Potential Mechanism of Clematichinenoside AR in Alleviating Hyperuricemia Based on Untargeted Metabolomics and Nrf2 Signaling Pathway"],"prefix":"10.1002","volume":"40","author":[{"given":"Qi","family":"Liang","sequence":"first","affiliation":[{"name":"Shandong Engineering Research Center for Innovation and Application of General Technology for Separation of Natural Products, Shandong Analysis and Test Center Qilu University of Technology (Shandong 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