{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T06:49:00Z","timestamp":1785739740771,"version":"3.56.0"},"reference-count":46,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2026,6,14]],"date-time":"2026-06-14T00:00:00Z","timestamp":1781395200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,14]],"date-time":"2026-06-14T00:00:00Z","timestamp":1781395200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2026,9,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Steroid hormone testing is critical for assessing endocrine function, diagnosing related disorders, and monitoring therapeutic efficacy. However, current mainstream detection methods have limitations. Although liquid chromatography\u2013tandem mass spectrometry (LC\u2013MS\/MS), regarded as the gold standard, offers high sensitivity and specificity, it involves complex and time\u2010consuming procedures. Immunoassays such as enzyme\u2010linked immunosorbent assay (ELISA) are simple and fast but are limited by poor throughput for multitarget detection. Therefore, it is crucial to develop an analytical method that streamlines procedures and enables efficient parallel detection of multiple targets.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We developed an integrated signal\u2010enhanced aptasensor platform for pooled MS detection of three steroid hormones in serum. It combines aptamer recognition with mass\u2010tag amplification. This approach uses biorecognition instead of chromatography, while mass spectrometry enables simultaneous readout of multiple mass tags from combined samples.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The method was evaluated by detecting three steroid hormones (vitamin D, cortisol, and testosterone) in simulated serum samples and human serum samples. The limits of detection (LODs) for these analytes ranged from 0.411 to 6.796\u2009nM, which are below the established clinical cut\u2010off values for each steroid, demonstrating the requisite sensitivity for detection.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This integrated signal\u2010enhanced aptasensor outperforms conventional LC\u2013MS\/MS in efficiency and ELISA in throughput, enabling the quantification of multiple serum steroid hormones. Therefore, we believe that this method could be potentially useful in the clinical screening of hormone\u2010related disorders and suitable for the analysis of serum.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.70121","type":"journal-article","created":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T06:28:14Z","timestamp":1781504894000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrating Aptasensor With Mass\u2010Tag Signal Amplification Strategy for the Detection of Three Steroid Hormones in Serum"],"prefix":"10.1002","volume":"40","author":[{"given":"Jia","family":"Zhao","sequence":"first","affiliation":[{"name":"Key Laboratory of Precision and Intelligent Chemistry University of Science and Technology of China  Hefei Anhui China"},{"name":"School of Chemistry and Materials Science University of Science and Technology of China  Hefei Anhui China"},{"name":"National Synchrotron 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 Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8391-4013","authenticated-orcid":false,"given":"Guangming","family":"Huang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Precision and Intelligent Chemistry University of Science and Technology of China  Hefei Anhui China"},{"name":"School of Chemistry and Materials Science University of Science and Technology of China  Hefei Anhui China"},{"name":"National Synchrotron Radiation Laboratory University of Science and Technology of China  Hefei Anhui 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