{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T11:11:20Z","timestamp":1788779480544,"version":"build-2803163510"},"reference-count":39,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:00:00Z","timestamp":1785283200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:00:00Z","timestamp":1785283200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22574105"],"award-info":[{"award-number":["22574105"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2026,10,30]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Comprehensive characterization of lignin\u2010derived oligomers remains a major analytical challenge because of their high structural diversity and the lack of methods capable of resolving native interunit linkages in complex biomass\u2010derived mixtures. Molecular\u2010level understanding of these oligomers is essential for elucidating lignin depolymerization mechanisms and guiding biomass valorization strategies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Lignin oligomers generated from methanol\u2010based flow\u2010through solvolysis of birch sawdust were analyzed by high\u2010performance liquid chromatography coupled with ultraviolet detection and tandem mass spectrometry (HPLC\u2010UVD\/MS\n                      <jats:sup>n<\/jats:sup>\n                      ). Chromatographic separation was performed on tandem reversed\u2010phase C18 columns, and structural characterization was achieved using electrospray ionization Orbitrap mass spectrometry with higher\u2010energy collisional dissociation (HCD).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Distinct chromatographic behaviors were observed for lignin\u2010derived products with different degrees of polymerization, enabling differentiation of monomeric and oligomeric species. Tandem MS analysis of representative oligomers revealed characteristic fragmentation pathways that enabled structural elucidation of oligomers. \u03b2\u2010O\u20104 linkages were identified as the predominant interunit structures, and native \u03b2\u2010\u03b2 motifs were also characterized.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Untargeted molecular\u2010level characterization of lignin\u2010derived products generated from methanol\u2010based flow\u2010through solvolysis of birch biomass provides direct insights into the composition and interunit linkages of native\u2010like lignin oligomers. These findings reveal the structural diversity of biomass\u2010derived oligomers and bridge the gap between model\u2010compound studies and real biomass\u2010derived lignin systems.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.70147","type":"journal-article","created":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T12:17:23Z","timestamp":1785327443000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular\u2010Level Characterization of Native Lignin Oligomers From Flow\u2010Through Solvolysis of Biomass by High\u2010Performance Liquid Chromatography\u2013Tandem Mass Spectrometry"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5576-1400","authenticated-orcid":false,"given":"Zhongyue","family":"Zhou","sequence":"first","affiliation":[{"name":"School of Mechanical Engineering Shanghai Jiao Tong University  Shanghai 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