{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T11:11:20Z","timestamp":1788779480136,"version":"build-2803163510"},"reference-count":27,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2026,10,30]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>\n                      SR9009 is a synthetic REV\u2010ERB agonist with potential performance\u2010enhancing properties and is included on the World Anti\u2010Doping Agency (WADA) Prohibited List for human sport. Its potential misuse has also raised concerns in animal sports, particularly equine and camel racing. Although SR9009 metabolism has been investigated in human and equine models, its metabolic fate in racing camels (\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Camelus dromedarius<\/jats:italic>\n                      <\/jats:styled-content>\n                      ) remains unclear. Characterizing its biotransformation pathways and identifying suitable biomarkers are essential for developing reliable anti\u2010doping screening strategies for camel racing.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      An in\u00a0vitro metabolic study was conducted using camel liver homogenate and the fungal model\n                      <jats:italic>Cunninghamella elegans<\/jats:italic>\n                      . Metabolites were characterized using liquid chromatography\u2010high\u2010resolution mass spectrometry (LC\u2010HRMS). Putative structural elucidation was achieved through accurate mass measurements of precursor and product ions. Diagnostic fragmentation pathways were established using collision\u2010induced dissociation (CID) tandem mass spectrometry (MS\/MS), enabling the systematic identification of metabolic modifications based on characteristic fragment ions and neutral losses.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Seventeen Phase I metabolites of SR9009 were identified. Major biotransformation pathways included N\u2010dealkylation, mono\u2010hydroxylation, di\u2010hydroxylation, and combinations thereof. The N\u2010dealkylated metabolite was the predominant metabolite detected in both camel liver homogenate and\n                      <jats:italic>Cunninghamella elegans<\/jats:italic>\n                      , indicating its suitability as a primary analytical target for anti\u2010doping screening. No Phase II metabolites were detected under the experimental conditions employed. Putative structural assignments were supported by characteristic high\u2010resolution tandem mass spectral fragmentation patterns.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This study provides the first comprehensive metabolic profile of SR9009 in a camel\u2010specific in\u00a0vitro model. The identification of 17 Phase I metabolites, particularly the predominant N\u2010dealkylated derivative, provides valuable analytical targets for doping control. These findings establish a metabolic foundation for future in\u00a0vivo studies to determine pharmacokinetics and detection windows in racing camels.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.70149","type":"journal-article","created":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T15:12:11Z","timestamp":1785424331000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["LC\u2010HRMS\u2010Based Metabolic Profiling of the REV\u2010ERB Agonist SR9009 in Camel Liver Homogenate and\n                    <i>Cunninghamella elegans<\/i>\n                    for Anti\u2010Doping Analysis"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-7501-0165","authenticated-orcid":false,"given":"Ansar Babu","family":"Palathinkal","sequence":"first","affiliation":[{"name":"Camel Forensic Laboratory Central Veterinary Research Laboratory  Dubai UAE"},{"name":"Post Graduate and Research Department of Chemistry Jamal Mohamed College (Affiliated to Bharathidasan University)  Tiruchirappal Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2764-7915","authenticated-orcid":false,"given":"S.","family":"Farook Basha","sequence":"additional","affiliation":[{"name":"Post Graduate and Research Department of Chemistry Jamal Mohamed College (Affiliated to Bharathidasan University)  Tiruchirappal Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8900-9735","authenticated-orcid":false,"given":"Saraswathy","family":"Laya","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Science United Arab Emirates University  Al Ain UAE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4926-0105","authenticated-orcid":false,"given":"Jahfar","family":"Nalakath","sequence":"additional","affiliation":[{"name":"Camel Forensic Laboratory Central Veterinary Research Laboratory  Dubai UAE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6876-7115","authenticated-orcid":false,"given":"O. K.","family":"Praseen","sequence":"additional","affiliation":[{"name":"Camel Forensic Laboratory Central Veterinary Research Laboratory  Dubai UAE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Joseph","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Diagnostic Section Central Veterinary Research Laboratory  Dubai UAE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shantymol V.","family":"Jose","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Diagnostic Section Central Veterinary Research Laboratory  Dubai UAE"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,30]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.7608.1"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aah4965"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg.2016.150"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2011.10.005"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2011.01.006"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1210\/endocr\/bqad069"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11030"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2017.02.006"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0162452"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms6759"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2015.11.086"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2021.114701"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2015.03.070"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcvm.2022.952114"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1904226116"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3213"},{"key":"e_1_2_11_18_1","unstructured":"WADA \u201cStandard Prohibited List \u201dhttps:\/\/www.wada\u2010ama.org\/sites\/default\/files\/2025."},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17101676"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.2538"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3124"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2024.116190"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.9633"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.3109\/00498254.2012.729102"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.2544"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.10111"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.7000"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.9916"}],"container-title":["Rapid Communications in Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rcm.70149","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/rcm.70149","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rcm.70149","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T10:40:47Z","timestamp":1788777647000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/rcm.70149"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,30]]},"references-count":27,"journal-issue":{"issue":"20","published-print":{"date-parts":[[2026,10,30]]}},"alternative-id":["10.1002\/rcm.70149"],"URL":"https:\/\/doi.org\/10.1002\/rcm.70149","archive":["Portico"],"relation":{},"ISSN":["0951-4198","1097-0231"],"issn-type":[{"value":"0951-4198","type":"print"},{"value":"1097-0231","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,30]]},"assertion":[{"value":"2026-05-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70149"}}